Improving multiphoton microscopy using annular beam shaping, focusing on imaging of human skin

Johan Borglin; Nicholas J. Durr; Stina Guldbrand; Onur Ferhanoglu; Ann-Marie Wennberg; Dag Hanstorp; Adela Ben-Yakar; Marica B. Ericson

doi:10.1117/12.2038942

28 February 2014 Improving multiphoton microscopy using annular beam shaping, focusing on imaging of human skin

Johan Borglin, Nicholas J. Durr, Stina Guldbrand, Onur Ferhanoglu, Ann-Marie Wennberg, Dag Hanstorp, Adela Ben-Yakar, Marica B. Ericson

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Proceedings Volume 8948, Multiphoton Microscopy in the Biomedical Sciences XIV; 89482K (2014) https://doi.org/10.1117/12.2038942
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Multiphoton fluorescence microscopy (MPM) is a method for high resolution, non-invasive investigations of biological tissue. The aim of introducing an annular shaped laser beam is to reduce the ouf-of-focus generated background signal improving imaging of light scattering tissue such as human skin. Simulations show that 50% of the beam radius can be blocked, while preserving the shape of the point spread function. Initial experiments performed on a phantom consisting of fluorescein and fluorescent beads embedded in agar by using a custom built MPM-set up show that by introducing a simple beam blocker to create an annular beam, the background signal is reduced with approximately 5%. Future work will include optimizing the set up, and creating phantoms with more light scattering properties.

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Johan Borglin, Nicholas J. Durr, Stina Guldbrand, Onur Ferhanoglu, Ann-Marie Wennberg, Dag Hanstorp, Adela Ben-Yakar, and Marica B. Ericson "Improving multiphoton microscopy using annular beam shaping, focusing on imaging of human skin", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89482K (28 February 2014); https://doi.org/10.1117/12.2038942

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