Digital Imaging Fluorescence Microscopy Reveals Intracellular Calcium Ions In Living Cardiac And Smooth Muscle Cells.

W Gil Wier; William F Goldman

doi:10.1117/12.945410

24 June 1988 Digital Imaging Fluorescence Microscopy Reveals Intracellular Calcium Ions In Living Cardiac And Smooth Muscle Cells.

W Gil Wier, William F Goldman

Author Affiliations +

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945410
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

We have used digital video microscopy to study the relationship of intracellular calcium ion concentration ([Ca2+]i) to the function of living cardiac and vascular smooth muscle cells. The technical goal of our work is to obtain, with high spatial and temporal resolution, "maps" of [Ca2+]i inside single living cells. To relate [Ca2+]i to cell function, such "maps" can be used in conjunction with measurements of cell electrical activity, contractile activity or biochemical assays.

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W Gil Wier and William F Goldman "Digital Imaging Fluorescence Microscopy Reveals Intracellular Calcium Ions In Living Cardiac And Smooth Muscle Cells.", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945410

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