Determination of nitric oxide mediating intracellular Ca2+ release on neurons based on confocal microscopy imaging

Liqin Zheng; Yuhua Wang; Yipeng He; Yixiu Zeng; Yanding Zhang; Shusen Xie

doi:10.1117/12.2068890

17 September 2014 Determination of nitric oxide mediating intracellular Ca²⁺ release on neurons based on confocal microscopy imaging

Liqin Zheng, Yuhua Wang, Yipeng He, Yixiu Zeng, Yanding Zhang, Shusen Xie

Author Affiliations +

Proceedings Volume 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014); 92301F (2014) https://doi.org/10.1117/12.2068890
Event: Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 2014, Wuhan, China

Abstract

The gas NO is a ubiquitous intercellular messenger that modulates a wide range of physiological and pathophysiological functions. But few studies were made to study the role of NO in the Ca²⁺ release in dorsal root ganglion (DRG) neurons by confocal microscopy. Thus the objective of this study was to assess if NO has a role in Ca²⁺ signaling in DRG neurons using confocal microscopy combined with special fluorescence probe Fluo-3/AM. A 100 μM concentration of the NO donors (Sodium Nitroprusside, Dihydrate, SNP) and NO synthase inhibitor (NG-Monomethyl-L-arginine, Monoacetate salt, L-NMMA) was used in the study. Results showed that the fluorescence intensity increased rapidly after injecting SNP, which indicated that SNP could enhance intracellular Ca²⁺ release. And the fluorescence intensity shrank gradually with time and kept at a low level for quite a long period after loading with L-NMMA which indicated that L-NMMA could block intracellular Ca²⁺ release. All these results demonstrated that NO was involved in the regulation of intracellular Ca²⁺ release in the DRG neurons.

Citation Download Citation

Liqin Zheng, Yuhua Wang, Yipeng He, Yixiu Zeng, Yanding Zhang, and Shusen Xie "Determination of nitric oxide mediating intracellular Ca²⁺ release on neurons based on confocal microscopy imaging", Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 92301F (17 September 2014); https://doi.org/10.1117/12.2068890

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