High-resolution stimulated Raman scattering microscopy by focal-field modulation

Jian Lin; Wei Zheng; Zhiwei Huang

doi:10.1117/12.2211978

14 March 2016 High-resolution stimulated Raman scattering microscopy by focal-field modulation

Jian Lin, Wei Zheng, Zhiwei Huang

Proceedings Volume 9712, Multiphoton Microscopy in the Biomedical Sciences XVI; 971220 (2016) https://doi.org/10.1117/12.2211978
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We report on a high-resolution stimulated Raman scattering (SRS) microscopy by phase modulation of the pump beam. In this study, an optimized phase pattern is applied to the pump beam by a spatial light modulator to minimize its focal spot size. Simulation shows that the central lobe of focused pump beam is reduced to around half of its original size, and the full-width at half-maximum of corresponding SRS distribution is reduced to less than 65% after focal-field modulation. We demonstrate this scheme by measuring the pump beam focal spot size and SRS imaging of PMMA beads.

Citation Download Citation

Jian Lin, Wei Zheng, and Zhiwei Huang "High-resolution stimulated Raman scattering microscopy by focal-field modulation", Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 971220 (14 March 2016); https://doi.org/10.1117/12.2211978

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