DETECTION OF LOW CONCENTRATION OF MATRIX METALLOPROTEINASE 9 (MMP9) USING RESONANT NANOPILLARS (R-NPS) AS LABEL-FREE OPTICAL TRANSDUCERS FOR DISEASE MONITORING

Ana Lopez; María-Fe Laguna; Beatriz Santamaria; Yolanda Ramirez; Pedro Herreros; Rocio López; Luca Tramarin; Sergio Quintero; Sidharam Pujari; Han Zuilhof; Nureddin Ashammakhi; Ali Khademhisseini; Miguel Holgado

doi:10.1117/12.2621967

7 March 2022 DETECTION OF LOW CONCENTRATION OF MATRIX METALLOPROTEINASE 9 (MMP9) USING RESONANT NANOPILLARS (R-NPS) AS LABEL-FREE OPTICAL TRANSDUCERS FOR DISEASE MONITORING

Ana Lopez, María-Fe Laguna, Beatriz Santamaria, Yolanda Ramirez, Pedro Herreros, Rocio López, Luca Tramarin, Sergio Quintero, Sidharam Pujari, Han Zuilhof, Nureddin Ashammakhi, Ali Khademhisseini, Miguel Holgado

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Proceedings Volume PC11953, Optical Fibers and Sensors for Medical Diagnostics, Treatment and Environmental Applications XXII; PC1195309 (2022) https://doi.org/10.1117/12.2621967
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Matrix metalloproteinase 9 (MMP9) is present in normal physiological events in the human body. However, it is also known for being involved in inflammation process related to different diseases, such as arthritis and metastasis. Thus, this protein can be used as a biomarker for diseases monitoring and therapies control and assessments. The goal of this study is to demonstrate that novel multiplexed R-NPs optical transducers independent arrays, (also called BICELLs, Biological Sensing Cells) can discriminate small concentrations of MMP9. We achieved a LoD of 84 ng/mL. This data can be improved by increasing the resolution of the characterization system and optimizing the biofunctionalization strategy. These results are promising and encourage us to perform prospective works addressed to replicate this experiment using a fluidic system for multiplexed real-time optical response, so as to facilitate the monitoring of the different selected biomarkers (thank to multiplexed configuration of R-NPS arrays), necessary to provide patients with a more accurate diagnosis and treatments, and therefore to boost up personalized medicine.

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Ana Lopez, María-Fe Laguna, Beatriz Santamaria, Yolanda Ramirez, Pedro Herreros, Rocio López, Luca Tramarin, Sergio Quintero, Sidharam Pujari, Han Zuilhof, Nureddin Ashammakhi, Ali Khademhisseini, and Miguel Holgado "DETECTION OF LOW CONCENTRATION OF MATRIX METALLOPROTEINASE 9 (MMP9) USING RESONANT NANOPILLARS (R-NPS) AS LABEL-FREE OPTICAL TRANSDUCERS FOR DISEASE MONITORING", Proc. SPIE PC11953, Optical Fibers and Sensors for Medical Diagnostics, Treatment and Environmental Applications XXII, PC1195309 (7 March 2022); https://doi.org/10.1117/12.2621967

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KEYWORDS

Multiplexing

Transducers

Biosensing

Dielectrics

Inflammation

Medicine

Optical arrays

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