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12 May 2022 Small particle imaging using lensfree holographic microscopy for protein sensing diagnostics
Euan McLeod, Zhen Xiong, Maryam Baker, Colin J. Potter, Weilin Liu
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Proceedings Volume PC11968, Optical Diagnostics and Sensing XXII: Toward Point-of-Care Diagnostics; PC1196801 (2022) https://doi.org/10.1117/12.2610516
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Bead-based assays are traditionally difficult to adapt for high-sensitivity quantitative point-of-care diagnostics. Here we use lensfree optical microscopes with automated image processing to quantitatively sense specific proteins in solution via the agglutination of functionalized beads within a microfluidic chip. Simple protocols and compact and inexpensive readout devices make our approach well-suited for point-of-care diagnostics. We sense interferon gamma, a biomarker of infectious and inflammatory disease, as well as NeutrAvidin. Furthermore, we discuss computational methods for improving the identification of small particles in the lensfree images, including sparsity-promoting regularized reconstruction and vectorial Green’s function modeling based on dipole electromagnetic scattering theory.
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Euan McLeod, Zhen Xiong, Maryam Baker, Colin J. Potter, and Weilin Liu "Small particle imaging using lensfree holographic microscopy for protein sensing diagnostics", Proc. SPIE PC11968, Optical Diagnostics and Sensing XXII: Toward Point-of-Care Diagnostics, PC1196801 (12 May 2022); https://doi.org/10.1117/12.2610516
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KEYWORDS
Diagnostics
Particles
Proteins
Holography
Microscopy
Point-of-care devices
Microfluidics
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