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17 March 2023 Highly multiplexed 3D FISH imaging in the embryonic quail heart
Junwoo Suh, Yehe Liu, Andrew Rollins, Michiko Watanabe, Michael Jenkins M.D.
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Proceedings Volume PC12355, Diagnostic and Therapeutic Applications of Light in Cardiology 2023; PC123550D (2023) https://doi.org/10.1117/12.2650229
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Spatially resolving the molecular expression of multiple probes in 3D can help us understand complex biological processes. For instance, our group has linked abnormal shear stress patterns caused by regurgitant blood flow with resultant congenital heart defects (CHDs). Abnormal shear stress mapped on images of molecular expression gives additional context to developmentally critical pathways such as epithelial-mesenchymal transition (EMT). However, highly multiplexed 3D imaging is needed to accurately identify regions of interest in the looping heart. In this study, we demonstrate a sequential imaging of a library of HCR FISH probes.
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Junwoo Suh, Yehe Liu, Andrew Rollins, Michiko Watanabe, and Michael Jenkins M.D. "Highly multiplexed 3D FISH imaging in the embryonic quail heart", Proc. SPIE PC12355, Diagnostic and Therapeutic Applications of Light in Cardiology 2023, PC123550D (17 March 2023); https://doi.org/10.1117/12.2650229
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KEYWORDS
Heart
Stereoscopy
3D image processing
Multiplexing
3D modeling
Blood circulation
Hemodynamics
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