Test method for evaluating the photocytotoxic potential of fluorescence imaging products

Shruti Vig; Brandon Gaitan; Lucas Frankle; Miriam Adwan; Yu Chen; Rosalie Elespuru; Joshua Pfefer; Huang-Chiao Huang

doi:10.1117/12.2652255

17 March 2023 Test method for evaluating the photocytotoxic potential of fluorescence imaging products

Shruti Vig, Brandon Gaitan, Lucas Frankle, Miriam Adwan, Yu Chen, Rosalie Elespuru, Joshua Pfefer, Huang-Chiao Huang

Proceedings Volume PC12359, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXI; PC1235908 (2023) https://doi.org/10.1117/12.2652255
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Contrast-enhanced fluorescent imaging has the potential to improve patient outcomes. A critical challenge that has not been rigorously evaluated is the potential photocytoxicity of the fluorophores used for imaging procedures. In this study, we have used a modified 3T3 Neutral Red Uptake assessment strategy to evaluate 4 imaging dyes and 2 known photosensitizers generating reactive molecular species. Results shed light on the relative photocytotoxicity of tested agents and provide strong evidence on the effectiveness of the method. These methods will provide a foundation for standardizing photo-safety testing of clinical fluorescence imaging products.

Conference Presentation

Citation Download Citation

Shruti Vig, Brandon Gaitan, Lucas Frankle, Miriam Adwan, Yu Chen, Rosalie Elespuru, Joshua Pfefer, and Huang-Chiao Huang "Test method for evaluating the photocytotoxic potential of fluorescence imaging products", Proc. SPIE PC12359, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXI, PC1235908 (17 March 2023); https://doi.org/10.1117/12.2652255

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