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Histological analysis of tissues is the current gold standard utilized by pathologists in reviewing excised tumor specimen margins. However, as a result of time-consuming and labor-intensive pre-processing steps this approach leads to postponed post-operative feedback to surgeons resulting in worsened patient prognosis. In this work we introduce a combined ultraviolet confocal reflectance and photoacoustic remote sensing microscopy system that allows tightly optically sectioned high-resolution virtual histology imaging of freshly excised thick tissues label-free. By removing tissue preparation steps, this technique has real potential to translate post-operative feedback into the surgical suite.
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Nathaniel J. M. Haven, Matthew Martell, Jean Deschenes, Lashan Peiris, Nadia Giannakopoulos, Roger Zemp, "Combined ultraviolet confocal reflectance and photoacoustic remote sensing microscopy for generating virtual sectioned histology," Proc. SPIE PC12379, Photons Plus Ultrasound: Imaging and Sensing 2023, PC123791T (9 March 2023); https://doi.org/10.1117/12.2650881