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15 March 2023 Single-molecule super-resolution imaging of microfabricated 3D substrates for 3D cell culture (Conference Presentation)
Clément Cabriel, Margoth Córdova-Castro, Erwin Berenschot, Amanda Dávila-Lezema, Kirsten Pondman, Séverine Le Gac, Niels Tas, Arturo Susarrey-Arce, Ignacio Izeddin
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Proceedings Volume PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI; PC123860A (2023) https://doi.org/10.1117/12.2648989
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
The potential of micro-and nanofabricated samples as a platform to modulate cell behavior using 3D physical cues has shown tremendous interest in cell differentiation. Investigating cell behavior and organization at the molecular level requires advanced imaging techniques. We produced multiscale 3D substrates with glass fractal pyramids composed of several generations of octahedra of decreasing sizes, which allow direct observation of cells in 3D SMLM. We show how these samples can be fluorescently labeled and used as a self-referenced sample for calibration and resolution measurements. Moreover, we perform quantitative 3D SMLM on cells growing on such fractal substrates, observing spheroid-like behavior.
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Clément Cabriel, Margoth Córdova-Castro, Erwin Berenschot, Amanda Dávila-Lezema, Kirsten Pondman, Séverine Le Gac, Niels Tas, Arturo Susarrey-Arce, and Ignacio Izeddin "Single-molecule super-resolution imaging of microfabricated 3D substrates for 3D cell culture (Conference Presentation)", Proc. SPIE PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI, PC123860A (15 March 2023); https://doi.org/10.1117/12.2648989
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KEYWORDS
3D image processing
Super resolution
Microfabrication
Stereoscopy
3D metrology
Fractal analysis
Glasses
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