Quantitative measurement of reactive oxygen species <i>in vivo</i> by utilizing a novel method: chemiluminescence with an internal fluorescence as reference

Yanchun Wei; Da Xing; Shiming Luo; Liyong Yang; Qun Chen

doi:10.1117/1.3368688

1 March 2010 Quantitative measurement of reactive oxygen species in vivo by utilizing a novel method: chemiluminescence with an internal fluorescence as reference

Yanchun Wei, Da Xing, Shiming Luo, Liyong Yang, Qun Chen

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Journal of Biomedical Optics, Vol. 15, Issue 2, 027006 (March 2010). https://doi.org/10.1117/1.3368688

Abstract

Reactive oxygen species (ROS) produced by photodynamic therapy (PDT) is recorded in vivo using a chemiluminescence (CL)-based gated optical system. A novel approach is developed to utilize the fluorescence (FL) of the CL probe as an internal fluorescence to calibrate the observed CL on pharmacokinetics of the probe in situ. The results show that during an in vivo PDT session, the intensity of CL decreases significantly and the decaying of CL is governed by fast and slow time components. By comparing the temporal profile of FL to that of the corresponding CL, it is found that the slow component is mainly attributed to the probe pharmacokinetics, whereas the fast component is likely due to rapid oxygen consumption as a result of PDT treatment. With carefully selected criteria, it is possible to minimize the effect of probe pharmacokinetics. This significantly improves the monitoring method for practical applications.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Yanchun Wei, Da Xing, Shiming Luo, Liyong Yang, and Qun Chen "Quantitative measurement of reactive oxygen species in vivo by utilizing a novel method: chemiluminescence with an internal fluorescence as reference," Journal of Biomedical Optics 15(2), 027006 (1 March 2010). https://doi.org/10.1117/1.3368688

Published: 1 March 2010

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