Spectral imaging – acquisition of images within specific spectral intervals - is a powerful tool for optical diagnostics, able to provide objective data on various clinical parameters, e.g. abnormal content of biomolecules in pathologic tissues. Performance of diagnostics depends on the spectral selectivity of imaging; from this point, ultra-narrowband spectral line imaging appears well-suited for diagnostic applications. Two prototype devices for triple laser line imaging have been developed and tested in laboratory and clinical environments. Large area or whole-body skin spectral imaging device comprises vertically movable high-resolution camera coupled with a specific illumination unit - side-emitting optical fiber spiral that emits simultaneously three laser spectral lines at the wavelengths 450 nm, 520 nm and 628 nm. In the other device, conventional white broadband endoscopic illumination has been replaced by a combined three spectral line white illumination from a low power RGB laser-fiber system attached to the lighting channel of intranasal endoscope. Both prototypes undergo clinical validation; their design details and preliminary test results are reported and discussed.
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