Disorder strength is a metric that is used to measure cell stiffness. Through calculating disorder strength from cell phase images reconstructed from various QPI systems, we have discovered that this parameter differs by system used for acquisition. With our model, we demonstrate that disorder strength is highly dependent on image resolution. When corrected with our simulation model, disorder strength measurements from different QPI systems can be directly compared and enable cross-system analysis. Cell disorder strength infers important biophysical information on cellular stiffness of normal cells, carcinogen-exposed cells, and cancer cells.
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