Microsurgical testicular sperm extraction (micro-TESE) is a surgical procedure that extracts sperm directly from testicular tissue for patients suffering from non-obstructive azoospermia (NOA). A more accurate and non-invasive visualization method is needed for assisting surgeons to increase the success rate and decrease the damage to patients. In this study, we developed a customized spectrum domain optical coherence tomography(SD-OCT) system for visualization of sperm within seminiferous tubules in NOA rat model. Our results show a 92.78% for detection of pockets of sperm, demonstrating the potential for the use of OCT for improved guidance in micro-TESE.
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