Dr. Chika Nishimura Profile

Dr. Chika Nishimura

at Kyoto Univ

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 15 March 2023 Presentation + Paper

Migration correction technique using spatial information of neuronal images in fiber-inserted mouse under free-running behavior

Kairi Mine, Chika Nishimura, Tomohiko Hayakawa, Satoshi Yawata, Dai Watanabe, Masatoshi Ishikawa

Proceedings Volume 12365, 1236508 (2023) https://doi.org/10.1117/12.2652032

KEYWORDS: Neurons, Optical fibers, Image processing, Autofluorescence, Detection and tracking algorithms, Brain, Cameras, Video, Signal processing, Animals

Read Abstract +

By chronically implanting optical fibers and measuring each modality over time, the relationship between changes in animal behavior and changes in each modality can be clarified. While there are some challenges to the practical application of optical fiber. First, the video of neural activity obtained by optical fiber is a mixture of changes in fluorescent signals accompanying neural activity and the fiber’s autofluorescence; hence the obtained neural image is first smoothed by a Gaussian function. A minimum value image is created for the smoothed input image, which is used as the background image to compensate for autofluorescence. In addition, the optical fiber inserted into the brain of a mouse shifts its position as the mouse moves, because the measurement is performed under free-running conditions, hence the differential image using the input image is generated in the marker domain as a pre-processing step. Template matching and angle/position correction of the marker are applied to the signal domain in the actual processing section to obtain corrected neural activity data. As a result, the amount of cell migration significantly improved due to the cell migration correction. This algorithm increases the signal-to-noise ratio of the data, enables observation of the same neurons over a long period, such as two hours, and makes detecting neural activity more accurate with the correction. In the future, we plan to correspond with the obtained activity to video images of mouse behavior to be taken separately.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years