KEYWORDS: Tissues, Objectives, Image resolution, Light sources and illumination, Microscopy, Visualization, Lenses, In vivo imaging, Point spread functions, Optical microsystems
We have developed a compact scattering-based light sheet microscopy (sLSM) probe capable of imaging unstained tissues with cellular resolution. In the compact sLSM probe, a custom miniature objective lens was developed to achieve a high lateral resolution, large field of view (FOV), and small field curvature. The measured resolution of the custom objective was 1.65 to 1.97 μm across the FOV of ±1.0 mm. The compact probe had dimensions of 4 cm in width and height and 10 cm in length. The compact sLSM probe achieved an axial resolution better than 5.6 μm over a depth range of 206.2 μm and a lateral resolution of 1.9 μm. Preliminary results showed that the compact sLSM probe could visualize cellular details of fixed human anal epithelial tissues in a similar manner to a bench light sheet microscopy device using off-the-shelf objective lenses.
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