Dr. Eugene C. Lin Profile

Dr. Eugene C. Lin

at Vanderbilt Univ

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 4 March 2019 Presentation

Multiplexed surface-enhanced Raman immunoimaging in vivo with gold nanostars (Conference Presentation)

Yu-Chuan Ou, Joseph Webb, Christine O'Brien, Isaac Pence, Eugene Lin, Eden Paul, Ethan Lippmann, Anita Mahadevan-Jansen, Rizia Bardhan

Proceedings Volume 10894, 108940G (2019) https://doi.org/10.1117/12.2506699

KEYWORDS: Raman spectroscopy, Gold, Multiplexing, In vivo imaging, Surface enhanced Raman spectroscopy, Imaging spectroscopy, Receptors, Molecules, Tumors, Nanostructures

Read Abstract +

The interactions of programmed death protein-1 (PD-1) on T cells with its ligand, PD-L1, strongly contributes to an immunosuppressive microenvironment. Blockades of PD-L1 have shown long-term survival in patients. However, <30% of patients respond to PD-L1 blockade, in part due to inaccurate identification of PD-L1 expression. An unmet clinical need exists for noninvasive detection techniques. Surface-enhanced Raman spectroscopy (SERS) imaging mediated by gold nanostructures has gained interest as a pre-clinical noninvasive diagnostic tool due to the high spatial resolution, portability, low cost, and rapid analysis. In this work, we demonstrate the use of gold nanostars conjugated with Raman tags for multiplexed SERS and simultaneous diagnosis of PD-L1 and epidermal growth factor receptor (EGFR) in vivo. Nanostars conjugated with anti-PDL1 antibodies/DTNB Raman molecule, and nanostars conjugated with anti-EGFR antibodies/pMBA Raman molecules were concurrently introduced to mice. Longitudinal Raman analysis demonstrated maximum accumulation of nanostars occurred 6h post IV delivery when strong increases in SERS signals for both Raman tags were observed. Raman signals decreased by 30% when both targeted receptors were pre-blocked with antibody (IP), indicating both the sensitivity and specificity of our platform in distinguishing tumors with varied expression of PD-L1 and EGFR. Furthermore, ex-vivo Raman maps provided assessment of biomarker status with cellular-resolution, and nanostars distribution in tumors. Finally, gold contents in organs were quantified with inductively coupled plasma mass spectrometry to evaluate their pharmacokinetics and biodistribution. This work shows gold nanostars-mediated SERS imaging provides a quantitative measure of PD-L1 to allow predictive and personalized immunotherapies with minimal toxicities.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years