Transmembrane proteins and ion channels are important drug targets and have been explored as single molecule
sensors. For these proteins to function normally they must be integrated within lipid bilayers; however, the labor and
skill required to create artificial lipid bilayers have the limited the possible applications utilizing these proteins. In order
to reduce the complexity and cost of lipid bilayer formation and measurement, we have modified a previously published
lipid bilayer formation technique using mechanically contacted monolayers so that the process is automatable, requiring
minimal operator input. Measurement electronics are integrated with the fluid handling system, greatly reducing the time
and operator feedback characteristically required of traditional bilayer experiments. To demonstrate the biological
functionality of the resultant bilayers and the system's capabilities as a membrane platform, the ion channel gramicidin A
was incorporated and measured with this system.
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