KEYWORDS: Point spread functions, Fluorophores, Molecules, Microscopy, Cameras, Hyperspectral imaging, Engineering, Super resolution, Simulations, Signal to noise ratio
Microscopists can minimize the crosstalk between multiple fluorescent channels by using maximally distinctive spectrally sensitive engineered point spread functions (PSFs). We introduce Chernoff Information as a figure of merit for creating such distinctive PSFs for single molecule localization microscopy and the average probability of error in trying to distinguish between PSFs as an evaluation metric for the distinctiveness of multi-color PSFs. This approach can be extended for an arbitrary combination of fluorophore emission spectra and a preferred number of phase-manipulation segments of a phase mask, even at low signal-to-noise ratios.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.