Dr. Jesper J. Serbin Profile

Dr. Jesper J. Serbin

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Publications (10)

Proceedings Article | 23 December 2004 Paper

High-resolution direct-write femtosecond laser technologies

Jesper Serbin, Boris Chichkov

Proceedings Volume 5620, (2004) https://doi.org/10.1117/12.583144

KEYWORDS: Two photon polymerization, Polymers, Femtosecond phenomena, Photonic crystals, Glasses, Refractive index, Crystals, Scanning electron microscopy, Laser applications, Transmittance

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Proceedings Article | 14 November 2003 Paper

Three-dimensional nanostructuring of hybrid materials by two-photon polymerization

Jesper Serbin, Boris Chichkov, Ruth Houbertz

Proceedings Volume 5222, (2003) https://doi.org/10.1117/12.503792

KEYWORDS: Two photon polymerization, Polymers, Femtosecond phenomena, Polymerization, Liquids, Nanostructuring, Nanolithography, Absorption, Scanning electron microscopy, Ultraviolet radiation

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Proceedings Article | 16 October 2003 Paper

Inactivation of proteins by irradiation of gold nanoparticles with nano- and picosecond laser pulses

Gereon Huettmann, Benno Radt, Jesper Serbin, Reginald Birngruber

Proceedings Volume 5142, (2003) https://doi.org/10.1117/12.500525

KEYWORDS: Proteins, Gold, Particles, Picosecond phenomena, Nanoparticles, Absorption, Pulsed laser operation, Laser tissue interaction, Laser therapeutics, Gaussian beams

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Proceedings Article | 29 April 2003 Paper

Three-dimensional nanostructuring by two-photon polymerization of hybrid materials

Jesper Serbin, Boris Chichkov

Proceedings Volume 5118, (2003) https://doi.org/10.1117/12.498936

KEYWORDS: Two photon polymerization, Polymers, Femtosecond phenomena, Liquids, Polymerization, Nanostructuring, Nanolithography, Absorption, Photonic crystals, Fabrication

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Proceedings Article | 15 April 2003 Paper

Three-dimensional microfabrication with femtosecond laser pulses

Jesper Serbin, R. Houbertz, Carsten Fallnich, Boris Chichkov

Proceedings Volume 4941, (2003) https://doi.org/10.1117/12.468235

KEYWORDS: Two photon polymerization, Femtosecond phenomena, Liquids, Polymers, Fabrication, Photonic crystals, Microfabrication, Absorption, Microscopes, 3D microstructuring

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Proceedings Article | 19 February 2003 Paper

Medical and biological applications for ultrafast laser pulses

Holger Lubatschowski, Alexander Heisterkamp, Fabian Will, Ajoy Singh, Jesper Serbin, Andreas Ostendorf, Omid Kermani, R. Heermann, Herbert Welling, Wolfgang Ertmer

Proceedings Volume 4830, (2003) https://doi.org/10.1117/12.486505

KEYWORDS: Laser tissue interaction, Tissues, Laser ablation, Femtosecond phenomena, Laser dentistry, Cornea, Pulsed laser operation, Laser vision correction, Surgery, Laser cutting

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Proceedings Article | 1 April 2002 Paper

Ultrafast laser pulses for medical applications

Holger Lubatschowski, Alexander Heisterkamp, Fabian Will, Jesper Serbin, Thorsten Bauer, Carsten Fallnich, Herbert Welling, Wiebke Mueller, Burkard Schwab, Ajoy Singh, Wolfgang Ertmer

Proceedings Volume 4633, (2002) https://doi.org/10.1117/12.461386

KEYWORDS: Pulsed laser operation, Tissues, Laser tissue interaction, Laser ablation, Laser dentistry, Femtosecond phenomena, Cornea, Surgery, Laser cutting, Laser therapeutics

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Proceedings Article | 25 February 2002 Paper

Generation of periodic microstructures with femtosecond laser pulses

Hans Toenshoff, Andreas Ostendorf, Frank Korte, J. Serbin, Thorsten Bauer

Proceedings Volume 4426, (2002) https://doi.org/10.1117/12.456818

KEYWORDS: Femtosecond phenomena, Polymerization, Silica, Micromachining, Streak cameras, Polymers, Laser ablation, Metals, Dielectrics, Solids

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Proceedings Article | 26 October 2001 Paper

Laser-generated micro- and nanoeffects: inactivation of proteins coupled to gold nanoparticles with nano- and picosecond pulses

Benno Radt, Jesper Serbin, Bjoern Lange, Reginald Birngruber, Gereon Huettmann

Proceedings Volume 4433, (2001) https://doi.org/10.1117/12.446518

KEYWORDS: Proteins, Picosecond phenomena, Gold, Particles, Laser tissue interaction, Luminescence, Absorption, Temperature metrology, Nanoparticles, Photochemistry

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Background: Protein denaturation in the fs-ns time regime is of fundamental interest for high precision applications in laser tissue interaction. Conjugates of colloidal gold coupled to proteins are presented as a model system for investigating ultrafast protein denaturation. It is expected that irradiation of such conjugates in tissue using pico- up to nanosecond laser pulses could result in effects with a spatial confinement in the regime of single macromolecules up to organelles. Materials and Methods: Experiments were done with bovine intestinal alkaline phosphatase (aP) coupled to 15 nm colloidal Gold. This complex was irradiated at 527 nm/ 532 nm with a variable number of pico- and nanosecond pulses. The radiant exposure per pulse was varied from 2 to 50 mJ/cm² in the case of the picosecond pulses and 10 to 500 mJ/cm² in the case of the nanosecond pulses. Denaturation was detected as a loss of protein function with the help of the uorescence substrate 4MUP. Results and Discussion: Irradiation did result in a steady decrease of the aP activity with increasing radiant exposures and increasing number of pulses. Inactivations up to 80% using 35 ps pulses at 527 nm with 50 mJ/cm² and a complete inactivation induced by 16 ns pulses at 450 mJ/cm² are discussed. The induced temperature in the particles and the surrounding water was calculated using Mie's formulas for the absorption of the nanometer gold particles and an analytical solution of the equations for heat diffusion. The calculated temperatures suggest that picosecond pulses heat a molecular scaled area whereas nanosecond pulses could be used for targeting larger cellular compartiments. It is difficult to identify one of the possible damage mechanisms, i.e. thermal denaturation or formation of micro bubbles, from the dependance of the inactivation on pulse energy and number of applied pulses. Therefore experiments are needed to further elucidate the damage mechanisms. The observed inactivation dependencies on applied energy and radiant power can not be explained with one or two photon photochemistry. In conclusion, denaturing proteins irreversibly via nanoabsorbers using pico-/ nanosecond laser pulses is possible. The expected confinement of the heat to the nanoabsorbers suggests that denaturation of proteins with nanometer precision could be possible with this approach. However, the mechanism of protein inactivation, which is part of present investigations, is crucial for the precision of such nanoeffects.

Proceedings Article | 9 July 2001 Paper

Model system for investigating laser-induced subcellular microeffects

Gereon Huettmann, Jesper Serbin, Benno Radt, Bjoern Lange, Reginald Birngruber

Proceedings Volume 4257, (2001) https://doi.org/10.1117/12.434739

KEYWORDS: Proteins, Particles, Picosecond phenomena, Gold, Luminescence, Absorption, Laser tissue interaction, Diffusion, Laser applications, Water

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Background: Laser induced protein denaturation is of fundamental interest for understanding the mechanisms of laser tissue interaction. Conjugates of nanoabsorbers coupled to proteins are presented as a model system for investigating ultrafast protein denaturation. Irradiation of the conjugates using repetitive picosecond laser pulses, which are only absorbed by the nanoabsorbers, could result in effects with a spatial confinement of less than 100 nm. Materials and Methods: Experiments were done with bovine intestinal alkaline phosphates (aP) coupled to 15 nm colloidal gold. This complex was irradiated at 527 nm wavelength and 35 ps pulse width with a varying number of pulses ranging form one up to 10⁴. The radiant exposure per pulse was varied form 2 mJ/cm² to 50 mJ/cm². Denaturation was detected as a loss of protein function with the help of the fluorescence substrate 4MUP. Results and discussion: Irradiation did result in a steady decrease of the aP activity with increasing radiant exposures and increasing number of pulses. A maximal inactivation of 80% was reached with 10⁴ pulses and 50 mJ/cm² per pulse. The temperature in the particles and the surrounding water was calculated using Mie's formulas for the absorption of the nanometer gold particles and ana analytical solution of the equations for heat diffusion. With 50 mJ/cm², the particles are heated above the melting point of gold. Since the temperature calculations strongly depend on changes in the state of matter of the particles and water, a very sophisticated thermal model is necessary to calculate exact temperatures. It is difficult to identify one of the possible mechanisms, thermal denaturation, photochemical denaturation or formation of micro bubbles from the dependance of the inactivation on pulse energy and number of applied pulses. Therefore, experiments are needed to further elucidate the damage mechanisms. In conclusion, denaturing proteins irreversibly via nanoabsorbers using picosecond laser pulses is possible. The confinement of the heat to the nanoabsorbers when irradiating with picosecond pulses suggests that the denaturation of proteins could be possible with nanometer precision in cells with this approach. However, the mechanism of protein inactivation, which is part of present investigations, is crucial for the precision of such nanoeffects.

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