This talk will introduce a novel, recently developed, instrument that combines light sheet microscopy, optical trapping and multiplane imaging in a single platform capable of imaging 3D cultures of live cell over long time courses and mapping to these images the local mechanical properties of the material surrounding the cells. The presentation will highlight the wide-range of samples this instrument can study and discuss the analysis involved to evaluate the rheological properties of the material when either the trapping potential or the samples are non-isotropic.
We present a bespoke new instrument for micro-mechanical sensing and imaging of 3D cell cultures. Our instrument combines optical tweezers and multiplane imaging for 3D optical microrheology, with light sheet microscopy for 3D fluorescence imaging. This integrated system allows us to visualise cells in their 3D environment while also mapping the physical properties of the extracellular matrix local to the cells. Such a holistic approach will allow for a wide range of fundamental questions in the life sciences to be addressed by effectively sensing the micro-world from a cell’s perspective.
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