Microfluidic device detections of E. coli K12 in deionized (DI) water and E. coli in field water sample were
demonstrated through static light scattering of latex immunoagglutination using proximity optical fibers. This method is
a fully-automated, one-step detection, and requires neither sample pre-treatment nor cell culturing often required in
many on-chip detections. We have used highly carboxylated polystyrene submicron latex particles without surfactants to
enhance diffusional mixing and prevent non-specific bindings towards successful demonstration of latex
immunoagglutination in microfluidic device. Detection of E. coli was performed by taking microscopic images from the
view cell of a microfluidic device and counting the fractions of non-agglutinated and agglutinated particles. The limit of
detection (LOD) was ca. 150 CFU ml-1 with this method for both E. coli K12 in DI water and E. coli in field water
sample, indicating no non-specific bindings. Improved LOD of < 4.3 CFU ml-1 was achieved by measuring forward
static light scattering from microfluidic device, using proximity optical fibers and a USB-powered miniature
spectrometer. The total assay time for sample preparation (mostly dilutions) and on-chip assay (mostly injections and
short incubation time) was < 10 min.
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