TIRF-Microscopy is widely used in cell biology to monitor dynamic biomolecular events occurring at the cell membranes. However, this technic is limited by weak fluorescence signal and background noise degrading the spatial resolution. We present high sensitive fluorescent TIRF-M using glass coverslips coated with one-dimensional photonic crystal (1DPC). The latter is made of dielectric multilayers optimized to generate a large field enhancement under TIR illumination at its free interface. Using a TIRF-microscope, we successfully demonstrate the capability of these substrates to enhance the fluorescence signal as compared to classical glass coverslips over fluorescently labeled HIV-1 and SARS-CoV-2 virus-like-particles imaging.
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