Recently we have developed a novel slab optical waveguide (SOWG) spectroscopy utilizing glycerol drop on a thin glass plate for light introduction, with which in situ absorption spectra of adsorbed materials in UV-visible range were measured. This SOWG system has some advantages such as a low loss, a broad-band transparency, an easy coupling, and high sensitivity under monolayer coverage. Additionally, we have utilized indium-tin-oxide (ITO) electrode thin film on a SOWG (ITO-SOWG) for in situ observation of electrochemical reactions on electrode surface. We have observed the adsorption behavior of proteins and dyes on solid/liquid interfaces and obtain the electrochemical responses of them.
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