A novel ImageJ plugin is designed to extend the depth-of-field (DoF) by seamlessly fusing a series of multi-focus images, allowing for in-depth analysis. Moreover, it has been tested on multi-exposure image stacks, demonstrating its adeptness in preserving intricate details within both poorly and brightly illuminated regions of 3-D specimens. The significance of this capability becomes particularly apparent when dealing with images that exhibit a limited DoF and varying exposure settings under low signal-to-noise ratio conditions. The plugin’s effectiveness has been thoroughly validated through the processing and analysis of numerous image stacks featuring diverse diatom and cyanobacteria species. The proposed methodology incorporates a two-scale decomposition (TSD) scheme, complemented by the refinement of weight maps using edge-preserving filtering (EPF). This dual approach ensures the preservation of fine details in the fused image while simultaneously minimizing noise. Such innovations make this plugin a valuable tool for researchers and analysts working with complex image datasets.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.