Samples of liquid dispersions of 4 μm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis.
The experimental setup for multispectral nanoparticle tracking analysis is developed. The optical system for sample illumination in nanoparticle tracking analysis is designed that makes it possible to obtain images of particles in suspensions with a high signal-to-noise ratio. The results of the comparison of particle size distributions obtained by nanoparticle tracking analysis using developed experimental setup, dynamic light scattering method and scanning electron microscope are shown.
The experimental setup for determination of particle sizes by nanotracking is developed. It is shown the possibility to identify particles with different fluorescent labels using developed experimental setup. The first results of analysis for samples of polystyrene micro- and nanoparticles are obtained using developed experimental setup.
Samples of liquid dispersions of gold nanorods and nanostars with different size and shape were synthesized and studied based on dynamic light scattering, polarization measurements, electrophoretic light scattering.
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