Dr. Pierre Bon Profile

Dr. Pierre Bon

Permanent reasearcher at CNRS

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Author

Publications (17)

Proceedings Article | 18 June 2024 Presentation

Super-resolution label-free optical microscopy

Pierre Bon, Alberto Aguilar, Duc-Minh Ta

Proceedings Volume PC12996, PC1299612 (2024) https://doi.org/10.1117/12.3024412

KEYWORDS: Super resolution, Phase imaging, Confocal microscopy, Biological imaging, Super resolution microscopy, Phase interferometry, Tissues, Tissue optics, Tantalum, Particles

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Proceedings Article | 17 March 2023 Presentation

Visualization of laser-induced periodic Nano structures beyond optical-resolution by noninvasive optical method

Jose-Alberto Aguilar Mora, Alain Abou Khalil, David Pallarés Aldeiturriaga, Sedao Xxx, Cyril Mauclair, Pierre Bon

Proceedings Volume 12409, 124090T (2023) https://doi.org/10.1117/12.2648932

KEYWORDS: Visualization, Nanostructures, Microscopy, Diffraction, Super resolution, Scanning electron microscopy, Femtosecond phenomena, Zinc oxide, Reflectivity, Reflection

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Proceedings Article | 30 May 2022 Presentation

Deep tissue 3D single-particle tracking using self-interference in near-infrared

Karen Caicedo, Antony Lee, Pierre Bon, Laurent Cognet

Proceedings Volume PC12144, PC1214407 (2022) https://doi.org/10.1117/12.2621018

KEYWORDS: Tissues, Near infrared, Quantum dots, Wavefronts, Video, Tissue optics, Super resolution microscopy, Super resolution, Microscopy, Luminescence

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Proceedings Article | 13 October 2020 Presentation + Paper

Can phase masks extend depth-of-field in localization microscopy?

Olivier Lévêque, Caroline Kulcsár, Hervé Sauer, Antony Lee, Pierre Bon, Laurent Cognet, François Goudail

Proceedings Volume 11351, 113510E (2020) https://doi.org/10.1117/12.2558426

KEYWORDS: Microscopy, Image quality, Luminescence, Microscopes, Deconvolution, Imaging systems, Image acquisition, Biology, Molecules, Super resolution

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Proceedings Article | 24 April 2017 Presentation

Fast quantitative retardance imaging of biological samples using quandri-wave interferometry (Conference Presentation)

Sherazade Aknoun, Julien Savatier, Pierre Bon, Benoit Wattellier, Serge Monneret

Proceedings Volume 10068, 1006802 (2017) https://doi.org/10.1117/12.2251975

KEYWORDS: Tumors, Digital imaging, Biological research, Tissues, Birefringence, Interferometry, Shearing interferometers, Polarization, Skin, Collagen

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Proceedings Article | 27 April 2016 Presentation

Label-free three dimensional reconstruction of biological samples (Conference Presentation)

Sherazade Aknoun, Pierre Bon, Julien Savatier, Serge Monneret, Benoit Wattellier

Proceedings Volume 9718, 97182A (2016) https://doi.org/10.1117/12.2212921

KEYWORDS: Tomography, Tissue optics, 3D image processing, Phase imaging, Microscopes, Near infrared, Phase measurement, Image resolution, 3D metrology, Shearing interferometers

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Proceedings Article | 27 April 2016 Presentation

Fast quantitative retardance imaging of biological samples using quadri-wave interferometry (Conference Presentation)

Sherazade Aknoun, Pierre Bon, Julien Savatier, Serge Monneret, Benoit Wattellier

Proceedings Volume 9718, 97182L (2016) https://doi.org/10.1117/12.2212895

KEYWORDS: Tissues, Interferometry, Phase imaging, Microscopy, Birefringence, Anisotropy, Polarized microscopy, Polarimetry, Diagnostics, Collagen

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We describe the use of polarized spatially coherent illumination to perform linear retardance imaging and measurements of semi-transparent biological samples using a quantitative phase imaging technique [1]. Quantitative phase imaging techniques [2-5] are used in microscopy for the imaging of semi-transparent samples and gives information about the optical path difference (OPD). The strength of those techniques is their non-invasive (the sample is not labelled) and fast approach. However, this high contrast is non-specific and cannot be linked to specific properties of the sample. To overcome this limitation, we propose to use polarized light in combination with QPI. Indeed, anisotropy has been used to reveal ordered fibrous structures in biological samples without any staining or labelling with polarized light microscopy [6-8]. Recent studies have shown polarimetry as a potential diagnostic tool for various dermatological diseases on thick tissue samples [9]. Particularly, specific collagen fibers spatial distribution has been demonstrated to be a signature for the optical diagnosis and prognosis of cancer in tissues [10]. In this paper, we describe a technical improvement of our technique based on high-resolution quadri-wave lateral shearing interferometry (QWLSI) and liquid crystal retarder to perform quantitative linear birefringence measurements on biological samples. The system combines a set of quantitative phase images with different excitation polarizations to create birefringence images. These give information about the local retardance and orientation of biological anisotropic components. We propose using a commercial QWLSI [11] (SID4Bio, Phasics SA, Saint Aubin, France) directly plugged onto a lateral video port of an inverted microscope (TE2000-U, Nikon, Japan). We are able to take retardance images in less than 1 second which allows us to record dynamic phenomena (living cells study) and make high speed acquisitions to reconstruct tissues virtual slides. Results on living cells and comparisons between healthy and tumoral 10 µm thick skin tissues will be presented.

Proceedings Article | 27 April 2016 Presentation

Label-free three-dimensional reconstruction of biological samples (Conference Presentation)

Sherazade Aknoun, Pierre Bon, Julien Savatier, Serge Monneret, Benoit Wattellier

Proceedings Volume 9713, 97131L (2016) https://doi.org/10.1117/12.2212943

KEYWORDS: 3D image processing, Tomography, Tissue optics, Phase imaging, Microscopes, Near infrared, Phase measurement, Image resolution, 3D metrology, Shearing interferometers

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SPIE Journal Paper | 22 December 2015 Open Access

Living cell dry mass measurement using quantitative phase imaging with quadriwave lateral shearing interferometry: an accuracy and sensitivity discussion

Sherazade Aknoun, Julien Savatier, Pierre Bon, Frédéric Galland, Lamiae Abdeladim, Benoit Wattellier, Serge Monneret

JBO, Vol. 20, Issue 12, 126009, (December 2015) https://doi.org/10.1117/12.10.1117/1.JBO.20.12.126009

KEYWORDS: Image segmentation, Phase imaging, Phase measurement, Objectives, Blood, Microscopes, Interferometry, Refractive index, Calibration, Image processing algorithms and systems

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Proceedings Article | 23 May 2013 Paper

Quadriwave lateral shearing interferometry as a quantification tool for microscopy. Application to dry mass determination of living cells, temperature mapping, and vibrational phase imaging

Serge Monneret, Pierre Bon, Guillaume Baffou, Pascal Berto, Julien Savatier, Sherazade Aknoun, Hervé Rigneault

Proceedings Volume 8792, 879209 (2013) https://doi.org/10.1117/12.2021331

KEYWORDS: Wavefronts, Microscopy, Phase measurement, Phase imaging, Raman spectroscopy, Luminescence, Interferometry, Image filtering, Phase shifts, Microscopes

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Proceedings Article | 22 February 2013 Paper

Tomographic incoherent phase imaging, a diffraction tomography alternative for any white-light microscope

Pierre Bon, Shérazade Aknoun, Julien Savatier, Benoit Wattellier, Serge Monneret

Proceedings Volume 8589, 858918 (2013) https://doi.org/10.1117/12.2003426

KEYWORDS: Refractive index, Tomography, Microscopes, Diffraction, Phase imaging, Modulation transfer functions, Luminescence, Objectives, Image processing, Sensors

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Proceedings Article | 22 February 2013 Paper

Quantitative birefringence imaging of biological samples using quadri-wave interferometry

Sherazade Aknoun, Pierre Bon, Julien Savatier, Benoit Wattellier, Serge Monneret

Proceedings Volume 8587, 85871D (2013) https://doi.org/10.1117/12.2003622

KEYWORDS: Birefringence, Polarization, Refractive index, Interferometry, Collagen, Geometrical optics, Polarizers, Microscopes, Phase measurement, Radio propagation

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Proceedings Article | 22 February 2013 Paper

Supercritical scattering microscoy for quantitative phase in the vicinity of a lamella

Pierre Bon, Thomas Barroca, Sandrine Lévêque-Fort, Emmanuel Fort

Proceedings Volume 8589, 858916 (2013) https://doi.org/10.1117/12.2004130

KEYWORDS: Microscopes, Scattering, Microscopy, Phase measurement, Objectives, Cameras, Silica, Diffusion, Luminescence, Image processing

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Proceedings Article | 22 February 2013 Paper

Supercritical self-interference fluorescence microscopy for full-field membrane imaging

Thomas Barroca, Pierre Bon, Sandrine Lévêque-Fort, Emmanuel Fort

Proceedings Volume 8589, 858911 (2013) https://doi.org/10.1117/12.2003736

KEYWORDS: Luminescence, Microscopy, Microscopes, Point spread functions, Glasses, Objectives, Near field, Interfaces, Signal processing, Cameras

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SPIE Journal Paper | 6 July 2012 Open Access

Optical detection and measurement of living cell morphometric features with single-shot quantitative phase microscopy

Pierre Bon, Julien Savatier, Marine Merlin, Serge Monneret, Benoit Wattellier

JBO, Vol. 17, Issue 7, 076004, (July 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.7.076004

KEYWORDS: Luminescence, Image filtering, Optical testing, Phase measurement, Refractive index, Microscopes, Microscopy, Wavefronts, Biomedical optics, Optical filters

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Proceedings Article | 23 February 2011 Paper

Quadriwave lateral shearing interferometry for quantitative phase microscopy: coupling phase imaging and fluorescence imaging

Pierre Bon, Julien Savatier, Benoit Wattellier, Didier Marguet, Serge Monneret

Proceedings Volume 7904, 79040P (2011) https://doi.org/10.1117/12.873177

KEYWORDS: Luminescence, Phase measurement, Image filtering, Optical filters, Phase imaging, Microscopes, Cameras, Visualization, Proteins, Linear filtering

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Proceedings Article | 25 February 2010 Paper

Quadriwave lateral shearing interferometry for quantitative phase microscopy: applications to long-duration imaging

Pierre Bon, Benoit Wattellier, Serge Monneret, Didier Marguet

Proceedings Volume 7570, 75700E (2010) https://doi.org/10.1117/12.844930

KEYWORDS: Linear filtering, Phase measurement, Image filtering, Visualization, Microscopes, Interferometry, Microscopy, Digital holography, Phase interferometry, Wavefront sensors

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Showing 5 of 17 publications

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