Detection of nucleic acids has become a promising approach in diagnostics, but current methods often have limitations such as the need for expensive equipment or reduced sensitivity and specificity. Tuberculosis caused by M. tuberculosis is a widespread infectious disease that is also prone to developing antibiotic resistance. In this study, we introduce a novel method for detection and genotyping of M. tuberculosis using a biosensor based on a four-way junction (4WJ) DNA probe and plasmon Au nanospheres.
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