Dr. Qinglei Hu Profile

Dr. Qinglei Hu

at Huazhong Univ of Science and Technology

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 18 July 2023 Paper

Label-free blood analysis utilizing contrast-enhanced defocusing imaging with machine vision

D. Chen, N. Li, X. Liu, S. Zeng, X. Lv, L. Chen, Y. Xiao, Q. Hu

Proceedings Volume 12745, 1274505 (2023) https://doi.org/10.1117/12.2682246

KEYWORDS: Blood, Machine vision, Point-of-care devices, Biomedical engineering, Optical medical imaging, Thin film diagnostics

Read Abstract +

Blood analysis, through the complete blood count, remains the most fundamental medical test for diagnosing broad diseases. Even so, it is still limited to central laboratories with sophisticated facilities and skilled professionals. Here, we propose a simple, machine-vision-aided, label-free blood analysis technique via a regular microscope utilizing contrast-enhance defocus imaging, i.e., defocusing imaging under 415 nm, small aperture illumination. We have shown that this technique can simultaneously obtain leucocytes’ optical phase and erythrocytes’ spectrophotometric information, making it feasible to realize five-part leucocyte differential and hemoglobin quantification with machine vision. The reliability was verified by comparing the quantified results with clinical reference results, which indicates significant linear correlations (significance levels ⪅ 0.0001 and Pearson coefficients ⪆ 0.90). We also show that the virtual staining of the label-free blood cell images can be performed with a generative adversarial network to mimic conventional Wright-Giemsa images, facilitating this technique’s medical translation. This study reports a simple, easy-to-use, quick, reliable blood analysis technique that may lead to a reformation in the blood analysis field. We emphasize this technique’s great potential for early screening of various diseases, including anemia, leukemia, and neglected tropical diseases, especially in resource-limited settings.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years