Brillouin microscopy is a noncontact all-optical method for biomechanical imaging. Whilst spontaneous Brillouin scattering microscopy has been widely used in biology, stimulated Brillouin scattering (SBS) microscopy has been mainly used for the examination of materials. Recently, SBS microscopy has been demonstrated useful for the mechanical imaging of live Caenorhabditis elegans nematodes.
Here, we will present SBS microscopy of live NIH/3T3 cells, with no apparent photodamage for ~260mW optical power on the samples. We will discuss the importance of multicomponent spectral analysis of the SBS spectra acquired along with the high spectral resolution of the SBS microscope using information theoretic measures.
Single fluorescent particle tracking (SPT) is a technique often used in the biophysical sciences in order to explore the environment of soft matter [C Manzo et al, Rep Prog Phys 78, 124601 (2015); H Shen et al, Chem Rev 117, 7331–7376 (2017)]. SPT has typically been performed using widefield sample illumination and fluorescent spheres at low concentration, thus limiting the achievable tracking depth and the density of mapped particle trajectories within the sample (and hence the statistical accuracy with which particle motions can be analyzed).
In this talk, we propose to use a light sheet fluorescence microscope with photoactivatable carriers for high density SPT in thick samples with depth selectivity. In particular, we will demonstrate the usefulness of this approach for investigating the environments of heterogeneous soft materials, such as agarose gels, with improved statistical accuracy, and for providing precise depth information on the mechanical and dynamical properties of inhomogeneous soft matter. Furthermore, a discussion on the applicability of our method to probe material rheology at the nanometer-scale will be presented.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.