The present era of minimal invasive dentistry emphasizes the early detection and remineralization of initial enamel
caries. Ozone has been shown to reverse the initial demineralization before the integrity of the enamel surface is lost.
Nano-hydroxyapatite is a proven remineralizing agent for early enamel caries. In the present study, the effect of ozone in
enhancing the remineralizing potential of nano-hydroxyapatite on artificially demineralized enamel was investigated
using laser induced fluorescence. Thirty five sound human premolars were collected from healthy subjects undergoing
orthodontic treatment. Fluorescence was recorded by exciting the mesial surfaces using 325 nm He-Cd laser with 2 mW
power. Tooth specimens were subjected to demineralization to create initial enamel caries. Following which the
specimens were divided into three groups, i.e ozone (ozonated water for 2 min), without ozone and artificial saliva.
Remineralization regimen was followed for 3 weeks. The fluorescence spectra of the specimens were recorded from all
the three experimental groups at baseline, after demineralization and remineralization. The average spectrum for each
experimental group was used for statistical analysis. Fluorescence intensities of Ozone treated specimens following
remineralization were higher than that of artificial saliva, and this difference was found to be statistically significant
(P<0.0001). In a nutshell, ozone enhanced the remineralizing potential of nanohydroxyapatite, and laser induced
fluorescence was found to be effective in assessing the surface mineral changes in enamel. Ozone can be considered an
effective agent in reversing the initial enamel caries there by preventing the tooth from entering into the repetitive
restorative cycle.
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