Significance: Single-molecule localization-based super-resolution microscopy has enabled the imaging of microscopic objects beyond the diffraction limit. However, this technique is limited by the requirements of imaging an extremely large number of frames of biological samples to generate a super-resolution image, thus requiring a longer acquisition time. Additionally, the processing of such a large image sequence leads to longer data processing time. Therefore, accelerating image acquisition and processing in single-molecule localization microscopy (SMLM) has been of perennial interest.
Aim: To accelerate three-dimensional (3D) SMLM imaging by leveraging a computational approach without compromising the resolution.
Approach: We used blind sparse inpainting to reconstruct high-density 3D images from low-density ones. The low-density images are generated using much fewer frames than usually needed, thus requiring a shorter acquisition and processing time. Therefore, our technique will accelerate 3D SMLM without changing the existing standard SMLM hardware system and labeling protocol.
Results: The performance of the blind sparse inpainting was evaluated on both simulation and experimental datasets. Superior reconstruction results of 3D SMLM images using up to 10-fold fewer frames in simulation and up to 50-fold fewer frames in experimental data were achieved.
Conclusions: We demonstrate the feasibility of fast 3D SMLM imaging leveraging a computational approach to reduce the number of acquired frames. We anticipate our technique will enable future real-time live-cell 3D imaging to investigate complex nanoscopic biological structures and their functions.
Laser based radio communication system, i.e. OptoRadio, using Orthogonal M-ary PSK Modulation scheme is presented in this paper. In this scheme, when a block of data needs to be transmitted, the corresponding block of biorthogonal code is transmitted by means of multi-phase shift keying. At the receiver, two photo diodes are cross coupled. The effect is that the net output power due to ambient light is close to zero. The laser signal is then transmitted only into one of the receivers. With all other signals being cancelled out, the laser signal is an overwhelmingly dominant signal. The detailed design, bit error correction capabilities, and bandwidth efficiency are presented to illustrate the concept.
Free space laser communications provides wide bandwidth and high security capabilities to cellular backhaul network in order to successfully accomplish data communication between cell sites and NOC (Network Operation Center). For this application, an optical receiver is a critical component and needs to be designed to operate in sunlight and other ambient noise environments while providing reliable data transmission. In this paper, a method of Free Space Laser Communication along with a differential optical receiver is presented for the backhaul solution of 5G networks that provides high capacity, reliability, less deployment cost, and long distance reach. At the receiver, two photo diodes are cross coupled. The effect is that the net output power is close to zero. The laser signal is then transmitted only into one of the receivers. With all other signals being cancelled out, the laser signal is an overwhelmingly dominant signal. In the proposed configuration, two signals generating photo-receptors are arranged such that when they are opposed to one another, the effect is a cancellation, if and only if the both photo-receptors receive the same amount of input.
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