Ms. V. Bleu Knight Profile

V. Bleu Knight

Graduate Student at New Mexico State Univ

SPIE Involvement:

Author

Publications (2)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 27 March 2006 Paper

The accumulation of nontargeted quantum dots in cultured human embryonic kidney cells

V. Bleu Knight, Elba Serrano

Proceedings Volume 6096, 60960Y (2006) https://doi.org/10.1117/12.641259

KEYWORDS: Signal detection, Kidney, Tissues, Confocal microscopy, Quantum dots, Luminescence, Cadmium, Target detection, Metals, Nanocrystals

Read Abstract +

Quantum dots (QDs) could offer significant advantages in clinical settings due to their high photostability and quantum yield. We are investigating the uptake and compartmentalization of QDs by cells because these processes are not fully characterized and there is potential for heavy metal toxicity when semiconductor nanocrystals are sequestered. Here we demonstrate the intracellular accumulation of QDs in human embryonic kidney cells (HEK-293; ATCC) exposed to nontargeted (Qtracker 565nm; QDot Corp.) or targeted (Qtracker 565 Cell Labeling Kit; QDot Corp.) QDs. As expected, 10 nM targeted QDs (Lagerholm et al., 2004, Nano Letters, 4:2019-2022) accumulated in HEK-293 cells and normal human astrocytes (NHA; Cambrex Biosciences) after 1 hr, while nontargeted QDs (200 nM) could be detected after 24 hr in HEK-293 but not NHA. The uptake of 10 nM targeted QDs was greater than the uptake of 200 nM nontargeted QDs as confirmed by the number and intensity of puncta visible in HEK-293 cells imaged with confocal microscopy. QD uptake was not detected in two Xenopus kidney cell lines (XLK-WG and A6; ATCC) exposed to nontargeted QDs (10-500 nM) for 18 hours. Co-labeling of HEK-293 cultures with CellTracker Red CMTPX (Invitrogen) following QD uptake verified that QD accumulation does not affect cell viability. Differences in QD uptake between cell lines could be species-specific or due to different growth conditions. The unexpected accumulation of nontargeted QDs raises questions about the uptake mechanism and the intracellular location that are being investigated with TEM. Supported by NIH-NIDCD (DC003292) and NMSU-ADVANCE (NSF0123690) to EES.

Proceedings Article | 28 April 2005 Paper

Multiphoton imaging of quantum dot bioconjugates in cultured cells following Nd:YLF laser excitation

Elba Serrano, V. Knight

Proceedings Volume 5705, (2005) https://doi.org/10.1117/12.590877

KEYWORDS: Neodymium lasers, Quantum dots, Ultraviolet radiation, Tissues, Sensors, Ear, Luminescence, Microscopes, Confocal microscopy, Absorption

Read Abstract +

Quantum dot bioconjugates offer unprecedented opportunities for monitoring biological processes and molecular interactions in cells, tissues, and organs. We are interested in developing applications that permit investigation of physiological processes and cytoskeletal organization in live cells, and allow imaging of complex organs, such as the auditory and vestibular sensory structures of the inner ear. Multiphoton microscopy is a powerful technique for acquiring images from deep within a sample while reducing phototoxic effects of laser light exposure on cells. Previous studies have established that a solid-state Nd:YLF laser can be used to acquire two-photon and three-photon images from live cells while minimizing phototoxic side effects (Wokosin et al., 1996, Bioimaging, 4:208-214; Squirrell et al., 1999, Nature Biotechnology, 8:763-767). We present here the results of experiments using an all-solid-state Nd:YLF 1047 nm femtosecond laser (Microlase DPM1000) source to excite quantum dot bioconjugates. Cells were labeled with Qdot (Quantum Dot Corporation) bioconjugates or with Alexa Fluor (Molecular Probes) bioconjugates and then imaged with a BioRad 1024 confocal microscope configured for multiphoton imaging using internal or external (non-descanned) detectors. Results demonstrate that the Nd:YLF laser can be used to stimulate fluorescence emission of quantum dots and Alexa Fluor bioconjugates in cultured amphibian (Xenopus) and mammalian (rat, chinese hamster) cells. We conclude that the Nd:YLF laser is a viable excitation source that extends the applicability of quantum dots for investigation of biological processes using multiphoton microscopy.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years