Oral squamous cell carcinoma (OSCC) has been shown to spread locally and infiltrate adjacent bone or via the lymphatic system to the cervical lymph nodes. This usually necessitates a surgical neck dissection and either a local or segmental resection for bone clearance. While histopathology remains the gold standard for tissue diagnosis, several new diagnostic techniques are being developed that rely on physical and biochemical changes that mirror or precede malignant changes within tissue. The aim of this study was to compare findings of Elastic Scattering Spectroscopy (ESS) with histopathology on formalin-fixed specimens of both neck lymph node dissections and de-calcified archival bone from patients with OSCC. We wished to see if this technique could be used as an adjunct or alternative to histopathology in defining cervical nodal involvement and if it could be used to identify bone resection margins positive for tumour. 130 lymph nodes were examined from 13 patients. The nodes were formalin-fixed, bivalved and examined by ESS. The intensity of the spectrum at 4 points was considered for comparison; at 360nm, 450nm, 630nm and 690nm. 341 spectra were taken from the mandibular specimens of 21 patients, of which 231 spectra were taken from histologically positive sites and the rest were normal. The nodes and bone specimens were then routinely processed with haematoxylin and eosin-stained sections, examined histopathologically, and the results compared. Using Linear Discriminant Analysis (LDA) as a statistical method, a sensitivity of 98% and a specificity of 68% was obtained for the neck nodes and a sensitivity of 87% and a specificity of 80% for the bone margins.
Conference Committee Involvement (10)
Optical Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2018
27 January 2018 | San Francisco, California, United States
Optical Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology
28 January 2017 | San Francisco, California, United States
Optical Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology
13 February 2016 | San Francisco, California, United States
Optical Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology
7 February 2015 | San Francisco, California, United States
Optical Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology
1 February 2014 | San Francisco, California, United States
Head and Neck Optical Diagnostics
12 May 2013 | Munich, Germany
Optical Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology
2 February 2013 | San Francisco, California, United States
Optical Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology
21 January 2012 | San Francisco, California, United States
Optical Imaging, Therapeutics, and Advanced Technology in Otolaryngology and Head and Neck Surgery
22 January 2011 | San Francisco, California, United States
Advanced Technology and Instrumentation in Otolaryngology: Lasers, Optics, Radio Frequency, and Related Technology
23 January 2010 | San Francisco, California, United States
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