We describe a microfluidic device capable of trapping, isolating, and lysing individual cells in parallel using
dielectrophoretic forces and a system of PDMS channels and valves. The device consists of a glass substrate patterned
with electrodes and two PDMS layers comprising of the microfluidic channels and valve control channels. Individual
cells are captured by positive dielectrophoresis using the microfabricated electrode pairs. The cells are then isolated into
nanoliter compartments using pneumatically actuated PDMS valves. Following isolation, the cells are lysed open by
applying an electric field using the same electrode pairs. With the ability to capture and compartmentalize single cells
our device may be combined with analytical methods for in situ molecular analysis of cellular components from single
cells in a highly parallel manner.
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