Development of long axial range 3D single-particle tracking by PSF engineering using birefringent substrates

Shuho Nozue; Satoshi Habuchi

doi:10.1117/12.3023289

12 March 2024 Development of long axial range 3D single-particle tracking by PSF engineering using birefringent substrates

Shuho Nozue, Satoshi Habuchi

Author Affiliations +

Proceedings Volume 12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; 128480F (2024) https://doi.org/10.1117/12.3023289
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

We propose a new method to engineer point spread function (PSF) for 3D single-particle tracking using mica. The imaging system in this method uses a standard wide-field fluorescence microscope; the only difference from the ordinary is using a mica as a substrate to mount the sample instead of the coverslip. This approach would have advantages over easy-toimplement, wide axial range tracking capability, and multiplexity of tracking. We demonstrate 3D single-particle tracking in a homogeneous solution.

Citation Download Citation

Shuho Nozue and Satoshi Habuchi "Development of long axial range 3D single-particle tracking by PSF engineering using birefringent substrates", Proc. SPIE 12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, 128480F (12 March 2024); https://doi.org/10.1117/12.3023289

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