Near-infrared Raman spectroscopy for optical diagnosis of gastric precancer

Seng Khoon Teh; Wei Zheng; Khek Yu Ho; Ming Teh; Khay Guan Yeoh; Zhiwei Huang

doi:10.1117/12.755370

19 November 2007 Near-infrared Raman spectroscopy for optical diagnosis of gastric precancer

Seng Khoon Teh, Wei Zheng, Khek Yu Ho, Ming Teh, Khay Guan Yeoh, Zhiwei Huang

Proceedings Volume 6826, Optics in Health Care and Biomedical Optics III; 682625 (2007) https://doi.org/10.1117/12.755370
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

Raman spectroscopy is a molecular vibrational spectroscopic technique that is capable of optically probing the biomolecular changes associated with diseased transformation. The purpose of this study was to explore near-infrared (NIR) Raman spectroscopy for identifying precancer (dysplasia) from normal gastric mucosa tissues. High-quality Raman spectra in the range of 800-1800 cm^-1 can be acquired from gastric tissue within 5 seconds. Raman spectra showed significant differences between normal and dysplastic tissue, particularly in the spectral ranges of 850-900, 1,200-1,290 and 1,500-1,800 cm^-1 which contained signals related to hydroxyproline, amide III and amide I of proteins, and C=C stretching of lipids, respectively. The ratio of Raman intensities at 875 to 1,450 cm^-1 provided good differentiation between normal and dysplastic gastric tissue (unpaired Students' t-test, p<0.001), indicating that NIR Raman spectroscopy has a great potential for the non-invasive diagnosis of dysplasia in the stomach.

Citation Download Citation

Seng Khoon Teh, Wei Zheng, Khek Yu Ho, Ming Teh, Khay Guan Yeoh, and Zhiwei Huang "Near-infrared Raman spectroscopy for optical diagnosis of gastric precancer", Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 682625 (19 November 2007); https://doi.org/10.1117/12.755370

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