Detection of malignant lesions in vivo in the upper gastrointestinal tract using image-guided Raman endoscopy

Mads Sylvest Bergholt; Wei Zheng; Kan Lin; Khek Yu Ho; Khay Guan Yeoh; Ming Teh; Jimmy Bok Yan So; Zhiwei Huang

doi:10.1117/12.908652

31 January 2012 Detection of malignant lesions in vivo in the upper gastrointestinal tract using image-guided Raman endoscopy

Mads Sylvest Bergholt, Wei Zheng, Kan Lin, Khek Yu Ho, Khay Guan Yeoh, Ming Teh, Jimmy Bok Yan So, Zhiwei Huang

Author Affiliations +

Proceedings Volume 8219, Biomedical Vibrational Spectroscopy V: Advances in Research and Industry; 82190M (2012) https://doi.org/10.1117/12.908652
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Raman spectroscopy is a vibrational analytic technique sensitive to the changes in biomolecular composition and conformations occurring in tissue. With our most recent development of near-infrared (NIR) Raman endoscopy integrated with diagnostic algorithms, in vivo real-time Raman diagnostics has been realized under multimodal wide-field imaging (i.e., white- light reflectance (WLR), narrow-band imaging (NBI), autofluorescence imaging (AFI)) modalities. A selection of 177 patients who previously underwent Raman endoscopy (n=2510 spectra) was used to render two robust models based on partial least squares - discriminant analysis (PLS-DA) for esophageal and gastric cancer diagnosis. The Raman endoscopy technique was validated prospectively on 4 new gastric and esophageal patients for in vivo tissue diagnosis. The Raman endoscopic technique could identify esophageal cancer in vivo with a sensitivity of 88.9% (8/9) and specificity of 100.0% (11/11) and gastric cancers with a sensitivity of 77.8% (14/18) and specificity of 100.0% (13/13). This study realizes for the first time the image-guided Raman endoscopy for real-time in vivo diagnosis of malignancies in the esophagus and gastric at the biomolecular level.

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Mads Sylvest Bergholt, Wei Zheng, Kan Lin, Khek Yu Ho, Khay Guan Yeoh, Ming Teh, Jimmy Bok Yan So, and Zhiwei Huang "Detection of malignant lesions in vivo in the upper gastrointestinal tract using image-guided Raman endoscopy", Proc. SPIE 8219, Biomedical Vibrational Spectroscopy V: Advances in Research and Industry, 82190M (31 January 2012); https://doi.org/10.1117/12.908652

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KEYWORDS

Raman spectroscopy

Endoscopy

Cancer

In vivo imaging

Tissues

Esophagus

Diagnostics

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