Real-time depth-resolved Raman endoscopy for in vivo diagnosis of dysplasia in Barrett's esophagus

Mads Sylvest Bergholt; Wei Zheng; Khek Yu Ho; Khay Guan Yeoh; Ming Teh; Jimmy Bok Yan So; Zhiwei Huang

doi:10.1117/12.2003995

20 March 2013 Real-time depth-resolved Raman endoscopy for in vivo diagnosis of dysplasia in Barrett's esophagus

Mads Sylvest Bergholt, Wei Zheng, Khek Yu Ho, Khay Guan Yeoh, Ming Teh, Jimmy Bok Yan So, Zhiwei Huang

Proceedings Volume 8576, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII; 85760D (2013) https://doi.org/10.1117/12.2003995
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Raman spectroscopy is a vibrational analytic technique sensitive to the changes in biomolecular composition and conformations occurring in tissue. With our most recent development of depth-resolved near-infrared (NIR) Raman endoscopy integrated with on-line diagnostic algorithms, in vivo real-time epithelial diagnostics has been realized under multimodal wide-field imaging (i.e., white- light reflectance (WLR), narrow-band imaging (NBI), autofluorescence imaging (AFI)) modalities. A selection of 43 patients who previously underwent Raman endoscopy (n=146 spectra) was used to render a robust model based on partial least squares - discriminant analysis (PLS-DA) for diagnosis of dysplasia in Barrett’s esophagus. The Raman endoscopy technique was validated prospectively on 2 new esophageal patients for in vivo tissue diagnosis. The Raman endoscopic technique could identify esophageal high-grade dysplasia in vivo with an accuracy of 85.9% (sensitivity: 91.3% (21/23): specificity 83.3% (40/48)) on spectrum basis. This study realizes for the first time depth-resolved Raman endoscopy for real-time in vivo diagnosis of dysplasia in Barrett’s epithelium at the biomolecular level.

Citation Download Citation

Mads Sylvest Bergholt, Wei Zheng, Khek Yu Ho, Khay Guan Yeoh, Ming Teh, Jimmy Bok Yan So, and Zhiwei Huang "Real-time depth-resolved Raman endoscopy for in vivo diagnosis of dysplasia in Barrett's esophagus", Proc. SPIE 8576, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII, 85760D (20 March 2013); https://doi.org/10.1117/12.2003995

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