An integrated coherent anti-Stokes Raman scattering and multiphoton imaging technique for liver disease diagnosis

Jian Lin; Fake Lu; Wei Zheng; Hanry Yu; Colin Sheppard; Zhiwei Huang

doi:10.1117/12.908200

9 February 2012 An integrated coherent anti-Stokes Raman scattering and multiphoton imaging technique for liver disease diagnosis

Jian Lin, Fake Lu, Wei Zheng, Hanry Yu, Colin Sheppard, Zhiwei Huang

Author Affiliations +

Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 822625 (2012) https://doi.org/10.1117/12.908200
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Liver steatosis and fibrosis are two prevalence liver diseases and may eventually develop into hepatocellular carcinoma (HCC) Due to their prevalence and severity, much work has been done to develop efficient diagnostic methods and therapies. Nonlinear optical microscopy has high sensitivity and chemical specificity for major biochemical compounds, making it a powerful tool for tissue imaging without staining. In this study, three nonlinear microscopy imaging modalities are applied to the study of liver diseases in a bile duct ligation rat modal. CARS shows the distributions of fats or lipids quantitatively across the tissue; SHG visualizes the collagens; and TPEF reveals the morphology of hepatic cells. The results clearly show the development of liver steatosis and fibrosis with time, and the hepatic fat and collagen fibrils are quantified. This study demonstrates the ability of multimodal nonlinear optical microscopy for liver disease diagnosis, and may provide new insights into the understanding of the mechanisms of steatosis/fibrosis transformations at the cellular and molecular levels.

Citation Download Citation

Jian Lin, Fake Lu, Wei Zheng, Hanry Yu, Colin Sheppard, and Zhiwei Huang "An integrated coherent anti-Stokes Raman scattering and multiphoton imaging technique for liver disease diagnosis", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822625 (9 February 2012); https://doi.org/10.1117/12.908200

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