Multimodal nonlinear optical imaging of obesity-induced liver steatosis and fibrosis

Jian Lin; Fake Lu; Wei Zheng; Dean C. S. Tai; Hanry Yu; Colin Sheppard; Zhiwei Huang

doi:10.1117/12.873507

22 February 2011 Multimodal nonlinear optical imaging of obesity-induced liver steatosis and fibrosis

Jian Lin, Fake Lu, Wei Zheng, Dean C. S. Tai, Hanry Yu, Colin Sheppard, Zhiwei Huang

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 79031V (2011) https://doi.org/10.1117/12.873507
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Liver steatosis/fibrosis represents the major conditions and symptoms for many liver diseases. Nonlinear optical microscopy has emerged as a powerful tool for label-free tissue imaging with high sensitivity and chemical specificity for several typical biochemical compounds. Three nonlinear microscopy imaging modalities are implemented on the sectioned tissues from diseased livers induced by high fat diet (HFD). Coherent anti-Stokes Raman scattering (CARS) imaging visualizes and quantifies the lipid droplets accumulated in the liver, Second harmonic generation (SHG) is used to map the distribution of aggregated collagen fibers, and two-photon excitation fluorescence (TPEF) reveals the morphology of hepatic cells based on the autofluorescence signals from NADH and flavins within the hepatocytes. Our results demonstrate that obesity induces liver steatosis in the beginning stage, which may progress into liver fibrosis with high risk. There is a certain correlation between liver steatosis and fibrosis. This study may provide new insights into the understanding of the mechanisms of steatosis/fibrosis transformations at the cellular and molecular levels.

Citation Download Citation

Jian Lin, Fake Lu, Wei Zheng, Dean C. S. Tai, Hanry Yu, Colin Sheppard, and Zhiwei Huang "Multimodal nonlinear optical imaging of obesity-induced liver steatosis and fibrosis", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031V (22 February 2011); https://doi.org/10.1117/12.873507

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