Direct imaging of macrophage activation during PDT treatment

Sheng Song; Feifan Zhou; Wei R. Chen; Da Xing

doi:10.1117/12.919005

13 March 2012 Direct imaging of macrophage activation during PDT treatment

Sheng Song, Feifan Zhou, Wei R. Chen, Da Xing

Proceedings Volume 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011); 83290M (2012) https://doi.org/10.1117/12.919005
Event: Photonics and Optoelectronics Meetings 2011, 2011, Wuhan, China

Abstract

Mounting evidence describes a more complex progress of macrophage activation during photodynamic therapy (PDT), which performing distinct immunological functions and different physiologies on surrounding cells and tissues. Macrophage-targeted PDT has been applied in the selective killing of cells involved in inflammation and tumor. We have previously shown that PDT-mediated tumor cells apoptosis can induce a higher level immune response than necrosis, and enhance the macrophage activation. However, the molecular mechanism of macrophage activation during PDT-induced apoptotic cells (AC) still unclear. Here, we use confocal microscopy to image the phagocytosis of tumor cells by macrophages. We also observed that PDT-treated AC can activate Toll-like receptors (TLRs) which are present on macrophages surface. Besides, the increase in nitric oxide (NO) formation in macrophages was detected in real time by a laser scanning microscopy. This study provided more details for understanding the molecular mechanism of the immune response induced by PDT-treated AC.

Citation Download Citation

Sheng Song, Feifan Zhou, Wei R. Chen, and Da Xing "Direct imaging of macrophage activation during PDT treatment", Proc. SPIE 8329, Tenth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2011), 83290M (13 March 2012); https://doi.org/10.1117/12.919005

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