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13 March 2024 Quantitative monitoring of microplastics and lipid metabolism in live zebrafish via hyperspectral stimulated Raman scattering microscopy
Le Xin, Zhiwei Huang
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Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC128550M (2024) https://doi.org/10.1117/12.3008492
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
We utilize spectral focusing hyperspectral stimulated Raman scattering (SRS) imaging for longitudinally assessment of the interactions between live zebrafish and microplastics (MP) throughout the development process. Our study delves into the pathways of MP uptake and the size-dependent accumulation of MP within different zebrafish organs during various developmental phases. The altered lipid metabolic dynamics uncovered by Raman-tagged hyperspectral SRS imaging further reveal the hepatic inflammation and disruptions in energy metabolism within MP-exposed zebrafish, providing fresh insights on the potential impacts and fate of MP on diverse living organisms.
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Le Xin and Zhiwei Huang "Quantitative monitoring of microplastics and lipid metabolism in live zebrafish via hyperspectral stimulated Raman scattering microscopy", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128550M (13 March 2024); https://doi.org/10.1117/12.3008492
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KEYWORDS
Microscopy
Mode conditioning cables
Raman scattering
Biological imaging
Liver
Picosecond phenomena
Organisms
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