As interest grows in using three-dimensional (3D) tumor organoids as models for drug discovery and precision medicine, platforms for the reliable functional testing and analysis of these organoids are needed. Our group previously developed high-speed live cell interferometry (HSLCI), a powerful high-throughput functional drug screening platform which employs automated, repeated quantitative phase imaging (QPI) to longitudinally track drug-induced changes in biomass accumulation dynamics over time. Here, we present a method for the automated bioprinting of 3D tumor organoids coupled with real-time, highly parallel biomass quantification using HSLCI, and demonstrate its utility for drug screening.
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