Prof. Max J. H. Witjes Profile

Prof. Max J. H. Witjes

at Univ. Medical Ctr. Groningen

SPIE Involvement:

Author | Editor

SPIE Journal Paper | 15 November 2024 Open Access

Systematic comparison of fluorescence imaging in the near-infrared and shortwave-infrared spectral range using clinical tumor samples containing cetuximab-IRDye800CW

Bas Keizers, Thomas S. Nijboer, Christa A. van der Fels, Marius C. van den Heuvel, Gooitzen van Dam, Schelto Kruijff, Igle Jan de Jong, Max J. Witjes, Floris Voskuil, Dimitris Gorpas, Wesley Browne, Pieter van der Zaag

JBO, Vol. 30, Issue S1, S13708, (November 2024) https://doi.org/10.1117/12.10.1117/1.JBO.30.S1.S13708

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Proceedings Article | 18 June 2024 Presentation + Paper

A dual-aperture fluorescence ratio approach to minimize optical property heterogeneities for direct applications in intraoperative fluorescence-guided surgery

Cody Rounds, Jaron de Wit, Jasper Vonk, Jennifer Vorjohan, Sophia Nelson, Allyson Trang, Brooke Villinski, Kimberley Samkoe, Jovan Brankov, Floris Voskuil, Max J. Witjes, Kenneth Tichauer

Proceedings Volume 13009, 1300909 (2024) https://doi.org/10.1117/12.3022497

KEYWORDS: Optical properties, Fluorescence, Surgery, Resection, Tumors, Tissues, Simulations, Monte Carlo methods, Molecular imaging, Matrices

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Proceedings Article | 18 June 2024 Presentation + Paper

First clinical application of paired-agent imaging to intraoperatively detect cancer spread in excised sentinel lymph nodes from patients with head and neck cancer

Anjalika Sharma, T. Nijboer, Cody Rounds, Jovan Brankov, Floris Voskuil, Max J. Witjes, Kenneth Tichauer

Proceedings Volume 13009, 130090A (2024) https://doi.org/10.1117/12.3022515

KEYWORDS: Cancer, Tumors, 3D modeling, Lymph nodes, In vitro testing, Tumor growth modeling, Oncology, Neck, Matrices, Cancer detection

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Cancer continues to be a significant global health issue in the 21st century, presenting substantial risks to the health and well-being of individuals worldwide. Although there have been improvements in comprehending the molecular processes of the disease and creating treatments that specifically target it, a considerable portion of patients continue to encounter difficulties in attaining favorable results. Conventional two-dimensional (2D) cell cultures have been extensively used in cancer research. However, their inability to accurately mimic the intricate characteristics of tumors limits their effectiveness in predicting how anticancer treatments would perform in clinical settings. In order to overcome these restrictions, three-dimensional (3D) cell culture models, specifically multicellular spheroids, have arisen as promising tool for investigating cancer biology and therapeutic response. This work analyzes the development and growth dynamics of spheroids obtained from four distinct cancer cell lines: 9L-GFP, U251-RFP, A431, and FaDu. The stability and growth features of these spheroids were evaluated by culturing them using different cell counts and dilution ratios. Confocal microscopy was used to observe the formation of spheroids and measure their sizes for a duration of seven days. The results of our study reveal clear variations in growth patterns and stability profiles across the investigated cell lines. Notably, the 9L-GFP cell line demonstrates exceptional stability and continuous growth. The statistical analysis demonstrated that spheroids exhibited the most stability when the cell count was 25,000 cells and the dilution ratio was 1:3, as indicated by the high R-squared values. These findings highlight the significance of adjusting the number of cells and dilution ratios to ensure consistent and replicable spheroid formation. In summary, our study emphasizes the capacity of 3D spheroid models as effective instruments in cancer research and medication development, providing vital information about tumor biology and therapeutic responses in a context that closely resembles the physiological conditions.

Proceedings Article | 13 March 2024 Presentation + Paper

A dual-aperture fluorescence ratio approach to guide margin assessment intraoperatively in head and neck cancer surgery

Cody Rounds, Jaron de Wit, Jasper Vonk, Jennifer Vorjohan, Sophia Nelson, Brooke Villinski, Allyson Trang, Jovan Brankov, Floris Voskuil, Max J. Witjes, Kenneth Tichauer

Proceedings Volume 12825, 1282502 (2024) https://doi.org/10.1117/12.3002628

KEYWORDS: Fluorescence, Surgery, Imaging systems, Cancer, Resection, Image analysis

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Proceedings Article | 13 March 2024 Presentation + Paper

Ex vivo detection of oral squamous cell carcinoma spread to lymph nodes using paired-agent fluorescence optical tomography: path to clinical translation

Anjalika Sharma, Cody Rounds, T. Nijboer, Floris Voskuil, Max J. Witjes, Jovan Brankov, Kenneth Tichauer

Proceedings Volume 12825, 1282507 (2024) https://doi.org/10.1117/12.3001099

KEYWORDS: Cancer, Lymph nodes, Neck, Tumors, Head, 3D modeling, Tumor growth modeling, Surgery, Neodymium, Cancer detection

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SPIE Journal Paper | 17 January 2024 Open Access

Improved intraoperative identification of close margins in oral squamous cell carcinoma resections using a dual aperture fluorescence ratio approach: first in-human results

Cody C. Rounds, Jaron G. de Wit, Jasper Vonk, Jennifer Vorjohan, Sophia Nelson, Allyson Trang, Brooke Villinski, Kimberley S. Samkoe, Jovan G. Brankov, Floris J. Voskuil, Max J. H. Witjes, Kenneth M. Tichauer

JBO, Vol. 29, Issue 01, 016003, (January 2024) https://doi.org/10.1117/12.10.1117/1.JBO.29.1.016003

KEYWORDS: Fluorescence, Resection, Tumors, Surgery, Imaging systems, Statistical analysis, Pathology, Cancer detection, Fluorescence imaging, Tissues

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Proceedings Article | 15 March 2023 Presentation + Paper

Fluorescence mesoscopic imaging of whole lymph nodes for intraoperative sentinel lymph node biopsy procedures

Kenneth Tichauer, Cody Rounds, Veronica Torres, Chengyue Li, Hang Nguyen, Thom Nijboer, Floris Voskuil, Jovan Brankov, Max J. Witjes

Proceedings Volume 12361, 123610C (2023) https://doi.org/10.1117/12.2654231

KEYWORDS: Lymph nodes, Monte Carlo methods, Cancer, Biological imaging, Simulations, Surgery, Fluorescence, Cancer detection

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Proceedings Article | 15 March 2023 Presentation + Paper

Rapid, variable aperture approach to quantify depth of fluorescence in a heterogenous medium

Cody Rounds, Jaron de Wit, Jasper Vonk, Floris Voskuil, Jennifer Vorjohan, Sophia Nelson, Brooke Villinski, Allyson Trang, Max J. Witjes, Kenneth Tichauer

Proceedings Volume 12361, 1236105 (2023) https://doi.org/10.1117/12.2650919

KEYWORDS: Monte Carlo methods, Tissues, Optical properties, Natural surfaces, Light scattering, Surgery, Tumors, Resection, Photons, Fluorescence intensity

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Proceedings Article | 14 August 2019 Presentation

Epidermal growth factor receptor: targeted photoimmunotherapy is fluence rate dependent in-vivo (Conference Presentation)

Henriëtte de Bruijn, Wei Peng, Timo ten Hagen, Go van Dam, Jan L. Roodenburg, Max Witjes, Dominic Robinson

Proceedings Volume 11070, 110702T (2019) https://doi.org/10.1117/12.2525595

KEYWORDS: Tumors, In vivo imaging, Receptors, Photoimmunotherapy, Photodynamic therapy, Skin, Head, Neck, Cancer, Oncology

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SPIE Journal Paper | 4 June 2012 Open Access

In vivo quantification of photosensitizer concentration using fluorescence differential path-length spectroscopy: influence of photosensitizer formulation and tissue location

Sebastiaan A. H. de Visscher, Max J. Witjes, Slávka Kaščáková, Henricus J. Sterenborg, Dominic Robinson, Jan L. Roodenburg, Arjen Amelink

JBO, Vol. 17, Issue 06, 067001, (June 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.6.067001

KEYWORDS: Tissues, Tongue, Laser induced plasma spectroscopy, Liver, Luminescence, Tissue optics, Photodynamic therapy, In vivo imaging, Scattering, Fluorescence spectroscopy

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Proceedings Article | 2 March 2010 Paper

The detection of oral cancer using differential pathlength spectroscopy

H. J. C. M. Sterenborg, S. Kanick, S. de Visscher, M. Witjes, A. Amelink

Proceedings Volume 7548, 75481V (2010) https://doi.org/10.1117/12.847859

KEYWORDS: Cancer, Spectroscopy, Fiber optics, Tumor growth modeling, Biopsy, Double positive medium, Pathology, Diagnostics, Blood, Fiber optics tests

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Proceedings Article | 14 July 2009 Paper

In vivo quantification of mTHPC fluorescence in skinfold observation chamber using excitation and detection towards the near infrared region

Slávka Kascáková, Sebastiaan de Visscher, Bastiaan Kruijt, Henriëtte de Bruijn, Angélique van der Ploeg-van den Heuvel, Henricus J. C. Sterenborg, Max J. Witjes, Arjen Amelink, Dominic Robinson

Proceedings Volume 7380, 738063 (2009) https://doi.org/10.1117/12.822947

KEYWORDS: Luminescence, Tissues, Tissue optics, Absorption, Near infrared, In vivo imaging, Photodynamic therapy, Fluorescence spectroscopy, Light scattering, Scattering

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SPIE Journal Paper | 1 September 2004 Open Access

Clinical study for classification of benign, dysplastic, and malignant oral lesions using autofluorescence spectroscopy

Diana de Veld, Marina Skurichina, Max Witjes, Robert Duin, Henricus Sterenborg, Jan Roodenburg

JBO, Vol. 9, Issue 05, (September 2004) https://doi.org/10.1117/12.10.1117/1.1782611

KEYWORDS: Tissues, Spectroscopy, Principal component analysis, Tumors, Tongue, Luminescence, Tissue optics, Cancer, Algorithm development, Artificial neural networks

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Proceedings Article | 31 October 2001 Paper

Optical detection of (pre-)malignant lesions of the oral mucosa: autofluorescence characteristics of healthy mucosa

Diana de Veld, Max Witjes, Jan Roodenburg, Willem Star, Hericus Sterenborg

Proceedings Volume 4432, (2001) https://doi.org/10.1117/12.447135

KEYWORDS: Spectroscopy, Cancer, Tongue, Luminescence, Tissue optics, Databases, Mouth, Optical spectroscopy, In vivo imaging, Head

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Showing 5 of 14 publications

SPIE Conference Volume | 14 June 2018

Optical Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2018

Conference Committee Involvement (1)

Optical Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2018

27 January 2018 | San Francisco, California, United States

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