We present a new dynamic full-field optical coherence tomography (D-FF-OCT) imaging technique, which is self-referenced, vibration-insensitive and overcomes D-FF-OCT limitations near specular surfaces, named interface self-referencing D-FF-OCT (iSR-D-FF-OCT). In fibroblasts, retinal organoids, and corneal explants, we demonstrate imaging in the vicinity of reflective interfaces without fringe artefacts, in a live, label-free, non-invasive manner with sub-diffraction limited sectioning capacity within 3D samples. iSR-(D)-FF-OCT has a homogeneous contrast across the imaging field enabling flawless mosaicking. iSR-(D)-FF-OCT complements and is easily implemented on existing D-FF-OCT set-ups to cover a zero to several hundred micrometer depth range.
We present the first dynamic full field optical coherence tomography (D-FFOCT) independent module which can be easily integrated into any commercial microscope. Combining the whole setup with a commercial incubator,, we demonstrate three-dimensional live imaging experiments over time periods of minutes to hours to days keeping the sample in healthy conditions. We show timelapse high resolution live images of retinal explants and organoids in disease modeling applications.
We present interface self-referencing D-FF-OCT (iSR-D-FF-OCT), a new dynamic full-field optical coherence tomography (D-FF-OCT) imaging technique, which is self-referenced, vibration-insensitive and overcomes D-FF-OCT fringe artefact limitations near specular surfaces. iSR-(D)-FF-OCT complements and is easily implemented on existing D-FF-OCT set-ups to cover a zero to several hundred micrometer depth range. iSR-(D)-FF-OCT has a homogeneous contrast across the imaging field enabling flawless mosaicking. In fibroblasts, retinal organoids, and corneal explants, we demonstrate imaging in the vicinity of reflective interfaces without fringe artefacts, in a live, label-free, non-invasive manner with sub-diffraction limited sectioning capacity within 3D samples.
We present the first dynamic full field optical coherence tomography (D-FFOCT) module which can be easily integrated into a commercial microscope. Benefitting from the optical standardization of commercial microscopy, we demonstrate three-dimensional live imaging experiments over time periods of minutes to hours to days thanks to the use of an incubator, able to maintain cell culture conditions. We show timelapse high resolution live images of retinal explants and organoids in disease modeling applications.
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