Dr. Sergey A. Ermilov Profile

Dr. Sergey A. Ermilov

CEO at PhotoSound Technologies Inc

SPIE Involvement:

Author

Area of Expertise:

Biomedical Photoacoustics , Optical Trapping and Manipulation

Websites:

Company Website

Publications (67)

Proceedings Article | 12 March 2024 Presentation + Paper

Presentation + Paper

High-throughput photoacoustic tomography by integrated robotics and automation

Nathanael Marshall, Hans-Peter Brecht, Weylan Thompson, Dylan Lawrence, Vanessa Marshall, Samuel Toler, Stanislav Emelianov, Anthony Yu, Mark Anastasio, Umberto Villa, Joshua Maxwell, Sergey Ermilov

Proceedings Volume 12842, 128420I (2024) https://doi.org/10.1117/12.3005569

KEYWORDS: Imaging systems, Animals, Photoacoustic spectroscopy, Control systems, Spatial resolution, Animal model studies, Robotics, Acquisition tracking and pointing, Automation, Laser safety

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SPIE Journal Paper | 19 January 2024 Open Access

Open Access

Spatiotemporal image reconstruction to enable high-frame-rate dynamic photoacoustic tomography with rotating-gantry volumetric imagers

Refik Mert Cam, Chao Wang, Weylan Thompson, Sergey A. Ermilov, Mark A. Anastasio, Umberto Villa

JBO, Vol. 29, Issue S1, S11516, (January 2024) https://doi.org/10.1117/12.10.1117/1.JBO.29.S1.S11516

KEYWORDS: Image restoration, Tomography, Photoacoustic tomography, Imaging systems, Transducers, Data acquisition, Biomedical optics, Matrices, Image processing, Video

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SignificanceDynamic photoacoustic computed tomography (PACT) is a valuable imaging technique for monitoring physiological processes. However, current dynamic PACT imaging techniques are often limited to two-dimensional spatial imaging. Although volumetric PACT imagers are commercially available, these systems typically employ a rotating measurement gantry in which the tomographic data are sequentially acquired as opposed to being acquired simultaneously at all views. Because the dynamic object varies during the data-acquisition process, the sequential data-acquisition process poses substantial challenges to image reconstruction associated with data incompleteness. The proposed image reconstruction method is highly significant in that it will address these challenges and enable volumetric dynamic PACT imaging with existing preclinical imagers.AimThe aim of this study is to develop a spatiotemporal image reconstruction (STIR) method for dynamic PACT that can be applied to commercially available volumetric PACT imagers that employ a sequential scanning strategy. The proposed reconstruction method aims to overcome the challenges caused by the limited number of tomographic measurements acquired per frame.ApproachA low-rank matrix estimation-based STIR (LRME-STIR) method is proposed to enable dynamic volumetric PACT. The LRME-STIR method leverages the spatiotemporal redundancies in the dynamic object to accurately reconstruct a four-dimensional (4D) spatiotemporal image.ResultsThe conducted numerical studies substantiate the LRME-STIR method’s efficacy in reconstructing 4D dynamic images from tomographic measurements acquired with a rotating measurement gantry. The experimental study demonstrates the method’s ability to faithfully recover the flow of a contrast agent with a frame rate of 10 frames per second, even when only a single tomographic measurement per frame is available.ConclusionsThe proposed LRME-STIR method offers a promising solution to the challenges faced by enabling 4D dynamic imaging using commercially available volumetric PACT imagers. By enabling accurate STIRs, this method has the potential to significantly advance preclinical research and facilitate the monitoring of critical physiological biomarkers.

Proceedings Article | 7 April 2023 Presentation

Presentation

Low-rank matrix estimation-based spatiotemporal image reconstruction from few tomographic measurements per frame for dynamic photoacoustic computed tomography

Refik Mert Cam, Chao Wang, Weylan Thompson, Sergey Ermilov, Mark Anastasio, Umberto Villa

Proceedings Volume 12463, 124630R (2023) https://doi.org/10.1117/12.2654199

KEYWORDS: Photoacoustic tomography, Tomography, Image restoration, Image analysis, Tissue optics, Biomedical optics, Tissues, Data acquisition, Biological research, Tumors

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Proceedings Article | 9 March 2023 Presentation + Paper

Presentation + Paper

Dynamic image reconstruction to monitor tumor vascular perfusion in small animals using 3D photoacoustic computed-tomography imagers with rotating gantries

Refik Mert Cam, Chao Wang, Seonyeong Park, Weylan Thompson, Sergey Ermilov, Mark Anastasio, Umberto Villa

Proceedings Volume 12379, 123790F (2023) https://doi.org/10.1117/12.2647663

KEYWORDS: Photoacoustic tomography, Tumors, Imaging systems, Acoustics, Matrices, Tomography, 3D image reconstruction, Preclinical imaging, Iterative methods, Inverse imaging problems, Small animal whole body imaging

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SPIE Journal Paper | 7 March 2023 Open Access

Open Access

Characterizing a photoacoustic and fluorescence imaging platform for preclinical murine longitudinal studies

Weylan R. Thompson, Hans-Peter F. Brecht, Vassili Ivanov, Anthony M. Yu, Diego S. Dumani, Dylan J. Lawrence, Stanislav Y. Emelianov, Sergey A. Ermilov

JBO, Vol. 28, Issue 03, 036001, (March 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.3.036001

KEYWORDS: Spatial resolution, Imaging systems, Photoacoustic spectroscopy, In vivo imaging, Acquisition tracking and pointing, Fluorescence imaging, Vacuum chambers, Biological imaging, Cameras, Reconstruction algorithms

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Proceedings Article | 3 March 2022 Presentation + Paper

Presentation + Paper

A combined ultrasound and photoacoustic imaging platform for clinical research applications

Weylan Thompson, Hans-Peter Brecht, Sergey Ermilov, Vassili Ivanov

Proceedings Volume 11960, 119600K (2022) https://doi.org/10.1117/12.2610099

KEYWORDS: Spatial resolution, Imaging systems, Ultrasonography, Photoacoustic imaging, In vivo imaging, Signal detection, Video, Functional imaging, Doppler effect, Data acquisition

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Proceedings Article | 6 March 2021 Presentation

Presentation

Dynamic reconstruction of three-dimensional photoacoustic tomography from few projections

Chao Wang, Umberto Villa, Weylan Thompson, Seonyeong Park, Sergey Ermilov, Mark Anastasio

Proceedings Volume 11642, 116421A (2021) https://doi.org/10.1117/12.2585344

KEYWORDS: Acquisition tracking and pointing, Photoacoustic tomography, 3D metrology, Reconstruction algorithms, 3D image reconstruction, Tomography, Tissues, Tissue optics, Temporal resolution, Numerical simulations

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Proceedings Article | 17 February 2020 Presentation + Paper

Presentation + Paper

A preclinical small animal imaging platform combining multi-angle photoacoustic and fluorescence projections into co-registered 3D maps

Weylan Thompson, Anthony Yu, Diego Dumani, Jason Cook, Mark Anastasio, Stanislav Emelianov, Sergey Ermilov

Proceedings Volume 11240, 112400L (2020) https://doi.org/10.1117/12.2549088

KEYWORDS: Photoacoustic imaging, Fluorescence tomography, Contrast sensitivity, Photoacoustic tomography, Optical imaging, In vivo imaging

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Proceedings Article | 17 February 2020 Paper

Paper

Massive parallel ultrasound and photoacoustic PC-based system

Weylan Thompson, Hans Peter Brecht, Sergey Ermilov, Vassili Ivanov

Proceedings Volume 11240, 112404H (2020) https://doi.org/10.1117/12.2547377

KEYWORDS: Ultrasonography, Photoacoustic spectroscopy, Interfaces, Field programmable gate arrays, Data acquisition, Connectors, Visualization, Phased arrays

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Proceedings Article | 19 March 2019 Paper

Paper

Preclinical small animal imaging platform providing co-registered 3D maps of photoacoustic response and fluorescence

Diego Dumani, Anthony Yu, Weylan Thompson, Hans-Peter Brecht, Vassili Ivanov, Mark Anastasio, Jason Cook, Sergey Ermilov, Stanislav Emelianov

Proceedings Volume 10878, 108784Y (2019) https://doi.org/10.1117/12.2514489

KEYWORDS: Skin, Luminescence, Photoacoustic spectroscopy, Tissues, 3D image processing, Animal model studies, Preclinical imaging, Spatial resolution, In vivo imaging, Acquisition tracking and pointing

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Proceedings Article | 27 February 2019 Paper

Paper

Real-time photoacoustic data acquisition with a thousand parallel channels at hundreds frames per second

Vassili Ivanov, Hans Brecht, Sergey Ermilov

Proceedings Volume 10878, 108784Z (2019) https://doi.org/10.1117/12.2514488

KEYWORDS: Data acquisition, Clocks, Capacitance, Interfaces, Electromagnetic coupling, Amplifiers, Connectors, Field effect transistors, Signal to noise ratio, Human-machine interfaces

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Proceedings Article | 16 March 2018 Presentation + Paper

Presentation + Paper

A 3D imaging system integrating photoacoustic and fluorescence orthogonal projections for anatomical, functional and molecular assessment of rodent models

Hans P. Brecht, Vassili Ivanov, Diego Dumani, Stanislav Emelianov, Mark Anastasio, Sergey Ermilov

Proceedings Volume 10494, 1049411 (2018) https://doi.org/10.1117/12.2290880

KEYWORDS: Imaging systems, Tissue optics, 3D image processing, In vivo imaging, Image resolution, Data acquisition

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Proceedings Article | 28 February 2018 Presentation + Paper

Presentation + Paper

Co-registered photoacoustic and fluorescent imaging of a switchable nanoprobe based on J-aggregates of indocyanine green

Diego Dumani, Hans-Peter Brecht, Vassili Ivanov, Ryan Deschner, Justin Harris, Kimberly Homan, Jason Cook, Stanislav Emelianov, Sergey Ermilov

Proceedings Volume 10494, 104942W (2018) https://doi.org/10.1117/12.2291262

KEYWORDS: Nanoprobes, Luminescence, Photoacoustic spectroscopy, Molecular imaging, Photoacoustic imaging, Indocyanine green, Preclinical imaging, 3D image processing, Photoacoustic tomography, Cancer

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SPIE Journal Paper | 8 November 2016 Open Access

Open Access

Imaging technique for real-time temperature monitoring during cryotherapy of lesions

Elena Petrova, Anton Liopo, Vyacheslav Nadvoretskiy, Sergey Ermilov

JBO, Vol. 21, Issue 11, 116007, (November 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.11.116007

KEYWORDS: Blood, Temperature metrology, Tissues, Thermometry, Calibration, Tissue optics, Real time imaging, Scattering, Statistical analysis, Thermography

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Proceedings Article | 29 March 2016 Paper

Paper

Advanced laser system for 3D optoacoustic tomography of the breast

Marc Klosner, Gary Chan, Chunbai Wu, Donald Heller, Richard Su, Sergey Ermilov, Hans Peter Brecht, Vassili Ivanov, Pratik Talole, Yang Lou, Mark Anastasio, Alexander Oraevsky

Proceedings Volume 9708, 97085B (2016) https://doi.org/10.1117/12.2209398

KEYWORDS: Breast, Laser systems engineering, Absorption, Imaging systems, Pulsed laser operation, Alexandrite lasers, Tissues, Blood, Breast imaging, Optical parametric oscillators

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Proceedings Article | 18 March 2016 Presentation + Paper

Presentation + Paper

Small animal optoacoustic tomography system for molecular imaging of contrast agents

Richard Su, Anton Liopo, Sergey Ermilov, Alexander Oraevsky

Proceedings Volume 9708, 97081S (2016) https://doi.org/10.1117/12.2217635

KEYWORDS: Molecular imaging, Tomography, Imaging systems, Signal to noise ratio, Pre-clinical research, Indocyanine green, In vivo imaging, Spatial resolution, Preclinical imaging, Animal model studies, Absorption, Tumors, Blood, Image segmentation, Protactinium, Neodymium

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Proceedings Article | 15 March 2016 Presentation + Paper

Presentation + Paper

In vivo cryoablation of prostate tissue with temperature monitoring by optoacoustic imaging

Elena Petrova, Massoud Motamedi, Alexander Oraevsky, Sergey Ermilov

Proceedings Volume 9708, 97080G (2016) https://doi.org/10.1117/12.2211190

KEYWORDS: Prostate, Tissues, Ultrasonography, Temperature metrology, Blood, In vivo imaging, Prostate cancer, Image filtering, Data acquisition, Calibration

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Proceedings Article | 11 March 2015 Paper

Paper

Effect of rotating partial illumination on image reconstruction for optoacoustic breast tomography

Yang Lou, Vyacheslav Nadvoretskiy, Kun Wang, Sergey Emilov, Alexander Oraevsky, Mark Anastasio

Proceedings Volume 9323, 93233L (2015) https://doi.org/10.1117/12.2084909

KEYWORDS: Breast, Transducers, Monte Carlo methods, Image restoration, Absorption, Tomography, Acoustics, Mammography, Imaging systems, Data modeling

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Proceedings Article | 11 March 2015 Paper

Paper

Three-dimensional laser optoacoustic and laser ultrasound imaging system for biomedical research

Sergey Ermilov, Richard Su, Andre Conjusteau, Tanmayi Oruganti, Kun Wang, Fatima Anis, Mark Anastasio, Alexander Oraevsky

Proceedings Volume 9323, 93230N (2015) https://doi.org/10.1117/12.2085028

KEYWORDS: Imaging systems, Ultrasonography, 3D image processing, Absorption, Acoustics, Transducers, Laser systems engineering, Reconstruction algorithms, Kidney, Tomography

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Proceedings Article | 11 March 2015 Paper

Paper

Universal temperature-dependent normalized optoacoustic response of blood

Elena Petrova, Anton Liopo, Alexander Oraevsky, Sergey Ermilov

Proceedings Volume 9323, 93231Y (2015) https://doi.org/10.1117/12.2083594

KEYWORDS: Blood, Temperature metrology, Tissues, Absorbance, Calibration, Tissue optics, Ultrasonography, Plasma, Light scattering, Liquids

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Proceedings Article | 11 March 2015 Paper

Paper

Speed of sound and acoustic attenuation of compounds affected during optoacoustic monitoring of thermal therapies measured in the temperature range from 5°C to 60°C

Tanmayi Oruganti, Elena Petrova, Alexander Oraevsky, Sergey Ermilov

Proceedings Volume 9323, 93233I (2015) https://doi.org/10.1117/12.2083375

KEYWORDS: Temperature metrology, Blood, Acoustics, Plasma, Silicon, Tissues, Signal attenuation, Water, Therapeutics, Ultrasonography

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Proceedings Article | 11 March 2015 Paper

Paper

Accelerated iterative image reconstruction in three-dimensional optoacoustic tomography

Fatima Anis, Kun Wang, Richard Su, Sergey Ermilov, Alexander Oraevsky, Mark Anastasio

Proceedings Volume 9323, 932315 (2015) https://doi.org/10.1117/12.2084087

KEYWORDS: Image restoration, Reconstruction algorithms, Transducers, 3D image processing, Tomography, Imaging systems, Image quality, Algorithm development, 3D modeling, Image analysis

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Proceedings Article | 3 March 2014 Paper

Paper

Controlled bacteria-gold nanorod interactions for enhancement of optoacoustic contrast

Anton Liopo, Paul Derry, Boris Ermolinsky, Richard Su, André Conjusteau, Sergey Ermilov, Eugene Zubarev, Alexander Oraevsky

Proceedings Volume 8943, 894368 (2014) https://doi.org/10.1117/12.2044628

KEYWORDS: Bacteria, Gold, Plasmons, Imaging systems, Absorbance, Scanning electron microscopy, Absorption, Optical properties, Calibration, Nanorods

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Proceedings Article | 3 March 2014 Paper

Paper

Investigation of the adjoint-state method for ultrasound computed tomography: a numerical and experimental study

Fatima Anis, Yang Lou, André Conjusteau, Richard Su, Tanmayi Oruganti, Sergey Ermilov, Alexander Oraevsky, Mark Anastasio

Proceedings Volume 8943, 894337 (2014) https://doi.org/10.1117/12.2042636

KEYWORDS: Ultrasonography, Reconstruction algorithms, Computed tomography, Imaging systems, Breast imaging, Biomedical optics, Transducers, CT reconstruction, Image restoration, Tomography

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Proceedings Article | 3 March 2014 Paper

Paper

Temperature dependence of Grüneisen parameter in optically absorbing solutions measured by 2D optoacoustic imaging

Elena Petrova, Sergey Ermilov, Richard Su, Vyacheslav Nadvoretskiy, André Conjusteau, Alexander Oraevsky

Proceedings Volume 8943, 89430S (2014) https://doi.org/10.1117/12.2039949

KEYWORDS: Temperature metrology, Nickel, Absorbance, Thermodynamics, Carbon, Thermography, Liquids, Optical testing, Fiber lasers, Laser optics

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Proceedings Article | 3 March 2014 Paper

Paper

3D laser optoacoustic ultrasonic imaging system for research in mice (LOUIS-3DM)

Sergey Ermilov, Richard Su, Andre Conjusteau, Vassili Ivanov, Vyacheslav Nadvoretskiy, Tanmayi Oruganti, Pratik Talole, Fatima Anis, Mark Anastasio, Alexander Oraevsky

Proceedings Volume 8943, 89430J (2014) https://doi.org/10.1117/12.2044817

KEYWORDS: Ultrasonography, Imaging systems, Acoustics, 3D image processing, Transducers, Receivers, Tomography, Visualization, Reconstruction algorithms, Prototyping

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Proceedings Article | 4 March 2013 Paper

Paper

Generation of wide-directivity broadband ultrasound by short laser pulses

André Conjusteau, Vyacheslav Nadvoretskiy, Sergey Ermilov, Alexander Oraevsky

Proceedings Volume 8581, 85814U (2013) https://doi.org/10.1117/12.2008869

KEYWORDS: Ultrasonography, Spherical lenses, Acoustics, Detector arrays, Wavefronts, Imaging systems, Neodymium, Pulsed laser operation, Sensors, 3D image processing

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Proceedings Article | 4 March 2013 Paper

Paper

3D laser optoacoustic ultrasonic imaging system for preclinical research

Sergey Ermilov, André Conjusteau, Travis Hernandez, Richard Su, Vyacheslav Nadvoretskiy, Dmitri Tsyboulski, Fatima Anis, Mark Anastasio, Alexander Oraevsky

Proceedings Volume 8581, 85810N (2013) https://doi.org/10.1117/12.2008811

KEYWORDS: Ultrasonography, Imaging systems, Acoustics, 3D image processing, Tomography, Visualization, Signal attenuation, 3D modeling, Ultrasound tomography, Pre-clinical research

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Proceedings Article | 4 March 2013 Paper

Paper

Image reconstruction and system optimization for three-dimensional speed of sound tomography using laser-induced ultrasound

Fatima Anis, Richard Su, Vyacheslav Nadvoretsky, André Conjusteau, Sergey Ermilov, Alexander Oraevsky, Mark Anastasio

Proceedings Volume 8581, 85814T (2013) https://doi.org/10.1117/12.2008813

KEYWORDS: Imaging systems, Ultrasonography, Transducers, 3D image processing, Tomography, Image restoration, Receivers, Reconstruction algorithms, 3D modeling, Biomedical optics

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Proceedings Article | 4 March 2013 Paper

Paper

Towards non-invasive in vivo measurements of nanoparticle concentrations using 3D optoacoustic tomography

Dmitri Tsyboulski, Anton Liopo, Richard Su, Sergei Ermilov, Sergei Bachilo, R. Bruce Weisman, Alexander Oraevsky

Proceedings Volume 8581, 85814V (2013) https://doi.org/10.1117/12.2008897

KEYWORDS: Absorption, Nanoparticles, Spleen, Liver, Kidney, Luminescence, In vivo imaging, Tomography, Tissue optics, Tissues

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Proceedings Article | 4 March 2013 Paper

Paper

Clinical feasibility study of combined opto-acoustic and ultrasonic imaging modality providing coregistered functional and anatomical maps of breast tumors

Jason Zalev, Bryan Clingman, Remie Smith, Don Herzog, Tom Miller, A. Thomas Stavros, Sergey Ermilov, André Conjusteau, Dmitri Tsyboulski, Alexander Oraevsky, Kenneth Kist, N. Dornbluth, Pamela Otto

Proceedings Volume 8581, 858103 (2013) https://doi.org/10.1117/12.2009112

KEYWORDS: Ultrasonography, Tumors, Breast, Tissues, Blood, Biopsy, Cancer, Tissue optics, Absorption, Diagnostics

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SPIE Journal Paper | 15 June 2012 Open Access

Open Access

Three-dimensional optoacoustic imaging as a new noninvasive technique to study long-term biodistribution of optical contrast agents in small animal models

Richard Su, Sergey Ermilov, Anton Liopo, Alexander Oraevsky

JBO, Vol. 17, Issue 10, 101506, (June 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.10.101506

KEYWORDS: Blood, 3D image processing, Gold, Nanoparticles, Video, Kidney, Image segmentation, Absorption, 3D modeling, Biomedical optics

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Proceedings Article | 23 February 2012 Paper

Paper

Optoacoustic angiography of peripheral vasculature

Sergey Ermilov, Richard Su, Mario Zamora, Travis Hernandez, Vyacheslav Nadvoretsky, Alexander Oraevsky

Proceedings Volume 8223, 82230D (2012) https://doi.org/10.1117/12.911629

KEYWORDS: Blood, Angiography, Acoustics, Visualization, Blood circulation, Capillaries, Blood vessels, Diagnostics, Pulsed laser operation, Neodymium

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Proceedings Article | 23 February 2012 Paper

Paper

Clinical feasibility study of combined optoacoustic and ultrasonic imaging modality providing coregistered functional and anatomical maps of breast tumors

Jason Zalev, Don Herzog, Bryan Clingman, Tom Miller, Kenneth Kist, N. Carol Dornbluth, B. Michelle McCorvey, Pamela Otto, Sergey Ermilov, Vyacheslav Nadvoretsky, Andre Conjusteau, Richard Su, Dmitri Tsyboulski, Alexander Oraevsky

Proceedings Volume 8223, 82230A (2012) https://doi.org/10.1117/12.910968

KEYWORDS: Tissue optics, Blood, Breast, Tumors, Ultrasonography, Tissues, Imaging systems, Absorption, Ultrasonics, Functional imaging

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Proceedings Article | 23 February 2012 Paper

Paper

Optoacoustic 3D visualization of changes in physiological properties of mouse tissues from live to postmortem

Richard Su, Sergey Ermiliov, Anton Liopo, Alexander Oraevsky

Proceedings Volume 8223, 82230K (2012) https://doi.org/10.1117/12.910975

KEYWORDS: Blood, Tissues, Neodymium, Tomography, Organisms, Kidney, 3D visualizations, Hypoxia, Opacity, Visualization

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Proceedings Article | 15 June 2011 Paper

Paper

Optoacoustic tomography in preclinical research: in vivo distribution of highly purified PEG-coated gold nanorods

Richard Su, Anton Liopo, Hans-Peter Brecht, Sergey Ermilov, Kirill Larin, Alexander Oraevsky

Proceedings Volume 8089, 808902 (2011) https://doi.org/10.1117/12.889757

KEYWORDS: Liver, Gold, Absorption, Toxicity, Tomography, In vivo imaging, 3D image processing, Tissues, Kidney, Nanorods

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Proceedings Article | 11 April 2011 Paper

Paper

Image processing and analysis in a dual-modality optoacoustic/ultrasonic system for breast cancer diagnosis

Vyacheslav Nadvoretskiy, Sergey Ermilov, Hans-Peter Brecht, Richard Su, Alexander Oraevsky

Proceedings Volume 7899, 789909 (2011) https://doi.org/10.1117/12.877389

KEYWORDS: Tumors, Image analysis, Image filtering, Imaging systems, Breast, Image processing, Breast cancer, Image enhancement, Tissues, Ultrasonography

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Proceedings Article | 11 April 2011 Paper

Paper

Optoacoustic imaging system with improved collection efficiency

Dmitri Tsyboulski, André Conjusteau, Sergey Ermilov, Hans-Peter Brecht, Anton Liopo, Richard Su, Vyacheslav Nadvoretsky, Alexander Oraevsky

Proceedings Volume 7899, 78991D (2011) https://doi.org/10.1117/12.877723

KEYWORDS: Transducers, Ultrasonography, Wavefronts, Microscopy, Reflectors, Mirrors, Acoustics, Signal attenuation, Imaging systems, Spherical lenses

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Proceedings Article | 28 February 2011 Paper

Paper

Gold nanorod distribution in mouse tissues after intravenous injection monitored with optoacoustic tomography

Richard Su, Anton Liopo, Hans-Peter Brecht, Sergey Ermilov, Alexander Oraevsky

Proceedings Volume 7899, 78994B (2011) https://doi.org/10.1117/12.878794

KEYWORDS: Liver, Kidney, Gold, Spleen, Tissues, Nanoparticles, Tomography, In vivo imaging, Silver, Nanorods

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Proceedings Article | 28 February 2011 Paper

Paper

Optoacoustic sensor for nanoparticle linked immunosorbent assay (NanoLISA)

André Conjusteau, Anton Liopo, Dmitri Tsyboulski, Sergey Ermilov, William Elliott, Norman Barsalou, Saher Maswadi, Randolph Glickman, Alexander Oraevsky

Proceedings Volume 7899, 789910 (2011) https://doi.org/10.1117/12.879401

KEYWORDS: Gold, Sensors, Nanoparticles, Biosensors, Target detection, Ferroelectric polymers, Pulsed laser operation, Acoustics, Signal to noise ratio, Nanorods

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SPIE Journal Paper | 1 March 2010 Open Access

Open Access

Real-time optoacoustic monitoring and three-dimensional mapping of a human arm vasculature

Matthew Fronheiser, Sergey Ermilov, Hans-Peter Brecht, André Conjusteau, Richard Su, Ketan Mehta, Alexander Oraevsky

JBO, Vol. 15, Issue 02, 021305, (March 2010) https://doi.org/10.1117/12.10.1117/1.3370336

KEYWORDS: Arteries, Veins, Blood vessels, Visualization, Imaging systems, Video, Ultrasonography, Real time imaging, Skin, Blood circulation

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SPIE Journal Paper | 1 March 2010 Open Access

Open Access

Optoacoustic imaging of the prostate: development toward image-guided biopsy

Mohammad Yaseen, Sergey Ermilov, Hans-Peter Brecht, Richard Su, André Conjusteau, Matthew Fronheiser, Brent Bell, Massoud Motamedi, Alexander Oraevsky

JBO, Vol. 15, Issue 02, 021310, (March 2010) https://doi.org/10.1117/12.10.1117/1.3333548

KEYWORDS: Prostate, Tissues, Acoustics, Tissue optics, Signal detection, Biopsy, In vivo imaging, Imaging systems, Tumors, Signal processing

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Proceedings Article | 26 February 2010 Paper

Paper

In vivo 3D visualization of peripheral circulatory system using linear optoacoustic array

Sergey Ermilov, Hans-Peter Brecht, Matthew Fronheiser, Vyacheslav Nadvoretsky, Richard Su, Andre Conjusteau, Alexander Oraevsky

Proceedings Volume 7564, 756422 (2010) https://doi.org/10.1117/12.847933

KEYWORDS: Blood vessels, Arteries, Computer simulations, Veins, Absorption, Skin, 3D image processing, Blood, In vivo imaging, Laser beam diagnostics

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Proceedings Article | 24 February 2010 Paper

Paper

Toward functional imaging using the optoacoustic 3D whole-body tomography system

R. Su, H.-P. Brecht, S. Ermilov, V. Nadvoretsky, A. Conjusteau, A. Oraevsky

Proceedings Volume 7564, 756423 (2010) https://doi.org/10.1117/12.847044

KEYWORDS: Absorption, Nickel, Tomography, Imaging systems, 3D image processing, Scattering, Signal processing, Wavelets, Functional imaging, Filtering (signal processing)

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Proceedings Article | 24 February 2010 Paper

Paper

Real-time optoacoustic imaging of breast cancer using an interleaved two laser imaging system coregistered with ultrasound

Sergey Ermilov, Matthew Fronheiser, Vyacheslav Nadvoretsky, Hans-Peter Brecht, Richard Su, André Conjusteau, Ketan Mehta, Pamela Otto, Alexander Oraevsky

Proceedings Volume 7564, 75641W (2010) https://doi.org/10.1117/12.847946

KEYWORDS: Tumors, Breast, Breast cancer, Ultrasonics, Imaging systems, Ultrasonography, Blood vessels, Mammography, Deconvolution, Visualization

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Proceedings Article | 24 February 2010 Paper

Paper

Compensation for transducer effects in optoacoustic tomography

Kun Wang, Mark Anastasio, Sergey Ermilov, Hans-Peter Brecht, Richard Su, Alexander Oraevsky

Proceedings Volume 7564, 75640P (2010) https://doi.org/10.1117/12.842459

KEYWORDS: Transducers, Reconstruction algorithms, Tomography, Image restoration, 3D modeling, Ultrasonography, Image resolution, Data modeling, Optical tomography, Optical imaging

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Proceedings Article | 24 February 2010 Paper

Paper

Effects of optical energy distribution on the generation of acoustic plane waves

André Conjusteau, Sergey Ermilov, Hans-Peter Brecht, Alexander Oraevsky

Proceedings Volume 7564, 75641L (2010) https://doi.org/10.1117/12.846992

KEYWORDS: Acoustics, Pulsed laser operation, Sensors, Transducers, Signal detection, Signal attenuation, Ultrasonics, Wavefronts, Laser energy, Absorption

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SPIE Journal Paper | 1 November 2009 Open Access

Open Access

Whole-body three-dimensional optoacoustic tomography system for small animals

Hans-Peter Brecht, Richard Su, Matthew Fronheiser, Sergey Ermilov, André Conjusteau, Alexander Oraevsky

JBO, Vol. 14, Issue 06, 064007, (November 2009) https://doi.org/10.1117/12.10.1117/1.3259361

KEYWORDS: Tomography, Transducers, 3D image processing, Imaging systems, Opacity, Visualization, Video, Veins, Tissue optics, Kidney

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SPIE Journal Paper | 1 March 2009 Open Access

Open Access

Laser optoacoustic imaging system for detection of breast cancer

Sergey Ermilov, Tuenchit Khamapirad, André Conjusteau, Morton Leonard, Ron Lacewell, Ketan Mehta, Tom Miller, Alexander Oraevsky

JBO, Vol. 14, Issue 02, 024007, (March 2009) https://doi.org/10.1117/12.10.1117/1.3086616

KEYWORDS: Tumors, Breast, Transducers, Acoustics, Imaging systems, Tissue optics, Breast cancer, Mammography, Ultrasonography, Signal detection

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Proceedings Article | 25 February 2009 Paper

Paper

Characterization of optoacoustic transducers through the analysis of angular-dependent frequency response

André Conjusteau, Sergey Ermilov, Richard Su, Hans-Peter Brecht, Matthew Fronheiser, Alexander Oraevsky

Proceedings Volume 7177, 71770U (2009) https://doi.org/10.1117/12.812339

KEYWORDS: Transducers, Acoustics, Sensors, Calibration, Wavefronts, Ultrasonography, Signal attenuation, Pulsed laser operation, Image restoration, Signal to noise ratio

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Proceedings Article | 25 February 2009 Paper

Paper

Detection of gold-nanorod targeted pathogens using optical and piezoelectric optoacoustic sensors: comparative study

André Conjusteau, Saher Maswadi, Sergey Ermilov, Hans-Peter Brecht, Norman Barsalou, Randolph Glickman, Alexander Oraevsky

Proceedings Volume 7177, 71771P (2009) https://doi.org/10.1117/12.813434

KEYWORDS: Transducers, Acoustics, Sensors, Laser beam diagnostics, Gold, Signal detection, Signal generators, Target detection, Pathogens, Nanorods

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Proceedings Article | 25 February 2009 Paper

Paper

Optoacoustic 3D whole-body tomography: experiments in nude mice

Hans-Peter Brecht, Richard Su, Matt Fronheiser, Sergey Ermilov, André Conjusteau, Anton Liopo, Massoud Motamedi, Alexander Oraevsky

Proceedings Volume 7177, 71770E (2009) https://doi.org/10.1117/12.812319

KEYWORDS: Blood vessels, Linear filtering, Wavelets, Tomography, Electronic filtering, Transducers, Imaging systems, Tissue optics, 3D image processing, Alexandrite lasers

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Proceedings Article | 25 February 2009 Paper

Paper

Development of laser optoacoustic and ultrasonic imaging system for breast cancer utilizing handheld array probes

Sergey Ermilov, Matthew Fronheiser, Hans-Peter Brecht, Richard Su, André Conjusteau, Ketan Mehta, Pamela Otto, Alexander Oraevsky

Proceedings Volume 7177, 717703 (2009) https://doi.org/10.1117/12.812192

KEYWORDS: Breast, Transducers, Tumors, Acoustics, Ultrasonics, Imaging systems, 3D modeling, Signal processing, Visualization, Tissue optics

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We describe two laser optoacoustic imaging systems for breast cancer detection based on arrays of acoustic detectors operated manually in a way similar to standard ultrasonic breast imaging. The systems have the advantages of standard light illumination (regardless of the interrogated part of the breast), the ability to visualize any part of the breast, and convenience in operation. The first system could work in both ultrasonic and optoacoustic mode, and was developed based on a linear ultrasonic breast imaging probe with two parallel rectangular optical bundles. We used it in a pilot clinical study to provide for the first time demonstration that the boundaries of the tumors visualized on the optoacoustic and ultrasonic images matched. Such correlation of coregistered images proves that the objects on both images represented indeed the same tumor. In the optoacoustic mode we were also able to visualize blood vessels located in the neighborhood of the tumor. The second system was proposed as a circular array of acoustic transducers with an axisymmetric laser beam in the center. It was capable of 3D optoacoustic imaging with minimized optoacoustic artifacts caused by the distribution of the absorbed optical energy within the breast tissue. The distribution of optical energy absorbed in the bulk tissue of the breast was removed from the image by implementing the principal component analysis on the measured signals. The computer models for optoacoustic imaging using these two handheld probes were developed. The models included three steps: (1) Monte Carlo simulations of the light distribution within the breast tissue, (2) generation of optoacoustic signals by convolving N-shaped pressure signals from spherical voxels with the shape of individual transducers, and (3) back-projecting processed optoacoustic signals onto spherical surfaces for image reconstruction. Using the developed models we demonstrated the importance of the included spatial impulse response of the optoacoustic imaging system.

Proceedings Article | 28 February 2008 Paper

Paper

Data processing and quasi-3D optoacoustic imaging of tumors in the breast using a linear arc-shaped array of ultrasonic transducers

Sergey Ermilov, Reda Gharieb, Andre Conjusteau, Tom Miller, Ketan Mehta, Alexander Oraevsky

Proceedings Volume 6856, 685603 (2008) https://doi.org/10.1117/12.770321

KEYWORDS: Tumors, Breast, Transducers, Acoustics, Tissues, Wavelets, Signal processing, 3D image processing, Image resolution, Tomography

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Proceedings Article | 28 February 2008 Paper

Paper

Optoacoustic system for 3D functional and molecular imaging in nude mice

Matthew Fronheiser, Alan Stein, Don Herzog, Scott Thompson, Anton Liopo, Mohammad Eghtedari, Massoud Motamedi, Sergey Ermilov, Andre Conjusteau, Reda Gharieb, Ron Lacewell, Tom Miller, Ketan Mehta, Alexander Oraevsky

Proceedings Volume 6856, 68560R (2008) https://doi.org/10.1117/12.770461

KEYWORDS: Imaging systems, Tissues, Tumors, Sensors, Gold, Molecular imaging, Image resolution, 3D image processing, Tissue optics, Nanorods

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A three-dimensional laser optoacoustic imaging system was developed, which combines the advantages of optical spectroscopy and high resolution ultrasonic detection, to produce high contrast maps of optical absorbance in tissues. This system was tested in a nude mouse model of breast cancer and produced tissue images of tumors and vasculature. The imaging can utilize either optical properties of hemoglobin and oxyhemoglobin, which are the main endogenous tissue chromophores in the red and near-infrared spectral ranges, or exogenous contrast agent based on gold nanorods. Visualization of tissue molecules targeted by the contrast agent provides molecular information. Visulization of blood at multiple colors of light provides functional information on blood concentration and oxygen saturation. Optoacoustic imaging, using two or more laser illumination wavelengths, permits an assessment of the angiogenesis-related microvasculature, and thereby, an evaluation of the tumor stage and its metastatic potential. The optoacoustic imaging system was also used to generate molecular images of the malignancy-related receptors induced by the xenografts of BT474 mammary adenocarcinoma cells in nude mice. The development of the latter images was facilitated by the use of an optoacoustic contrast agent that utilizes gold nanorods conjugated to monoclonal antibody raised against HER2/neu antigens. These nanorods possess a very strong optical absorption peak that can be tuned in the near-infrared by changing their aspect ratio. The effective conversion of the optical energy into heat by the gold nanorods, followed by the thermal expansion of the surrounding water, makes these nanoparticles an effective optoacoustic contrast agent. Optical scattering methods and x-ray tomography may also benefit from the application of this contrast agent. Administration of the gold nanorod bioconjugates to mice resulted in an enhanced contrast of breast tumors relative the background of normal tissues in the nude mouse model. The combination of this novel contrast agent and optoacoustic imaging has the potential to become a useful imaging modality, for preclinical research in murine models of cancer and other human diseases.

Proceedings Article | 28 February 2008 Paper

Paper

Hybrid optoacoustic and ultrasonic imaging system for detection of prostate malignancies

Mohammad Yaseen, H. P.-F. Brecht, Sergey Ermilov, Reda Gharieb, Andre Conjusteau, Alexander Oraevsky

Proceedings Volume 6856, 68560T (2008) https://doi.org/10.1117/12.769881

KEYWORDS: Prostate, Tissues, Tumors, Signal detection, Ultrasonography, Transducers, Signal processing, Imaging systems, Ultrasonics, Absorption

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Proceedings Article | 15 February 2007 Paper

Paper

Detection and noninvasive diagnostics of breast cancer with 2-color laser optoacoustic imaging system

Sergey Ermilov, Alan Stein, Andre Conjusteau, Reda Gharieb, Ron Lacewell, Tom Miller, Scott Thompson, Pamela Otto, Barbara McCorvey, Tuenchit Khamapirad, Morton Leonard, Alexander Oraevsky

Proceedings Volume 6437, 643703 (2007) https://doi.org/10.1117/12.714039

KEYWORDS: Transducers, Tumors, Acoustics, Breast, Absorption, Imaging systems, Tissues, Breast cancer, Tomography, Signal processing

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Proceedings Article | 6 March 2006 Paper

Paper

128-channel laser optoacoustic imaging system (LOIS-128) for breast cancer diagnostics

Sergey Ermilov, Andre Conjusteau, Ketan Mehta, Ron Lacewell, P. Mark Henrichs, Alexander Oraevsky

Proceedings Volume 6086, 608609 (2006) https://doi.org/10.1117/12.658158

KEYWORDS: Transducers, Absorption, Tumors, Acoustics, Imaging systems, Diagnostics, Signal processing, Signal detection, Tissues, Breast

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Proceedings Article | 6 March 2006 Paper

Paper

Metallic nanoparticles as optoacoustic contrast agents for medical imaging

Andre Conjusteau, Sergey Ermilov, Dmitri Lapotko, Hongwei Liao, Jason Hafner, Mohammad Eghtedari, Massoud Motamedi, Nicholas Kotov, Alexander Oraevsky

Proceedings Volume 6086, 60860K (2006) https://doi.org/10.1117/12.658065

KEYWORDS: Nanoparticles, Gold, Tumors, Particles, Absorption, Plasmons, Laser damage threshold, Water, Cancer, Nanorods

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Proceedings Article | 6 March 2006 Paper

Paper

In vivo testing of laser optoacoustic system for image-guided biopsy of prostate

Alexander Oraevsky, Sergey Ermilov, Ketan Mehta, Tom Miller, Brent Bell, Eduardo Orihuela, Massoud Motamedi

Proceedings Volume 6086, 60860B (2006) https://doi.org/10.1117/12.658295

KEYWORDS: Prostate, Transducers, Blood, Tumors, Ultrasonography, Ultrasonics, Signal detection, Tissues, Reconstruction algorithms, Acoustics

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Proceedings Article | 18 October 2004 Paper

Paper

Measurements of cell wall mechanical properties using optically trapped fluorescent microspheres

Sergey Ermilov, Feng Qian, David Murdock, William Brownell, Bahman Anvari

Proceedings Volume 5514, (2004) https://doi.org/10.1117/12.559717

KEYWORDS: Phase modulation, Plasma, Optical tweezers, Luminescence, Ferroelectric materials, Mechanics, Visualization, Bandpass filters, Imaging systems, Cytoskeletons

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Information on plasma membrane (PM) and cell wall mechanical properties is important for many biophysical applications, especially for those, which involve cells, undergoing significant mechanical stress (red blood cells, outer hair cells, fibrocytes, etc.). Optical tweezers is frequently used to study PM mechanics, particularly by pulling long PM tethers. One of the limitations on using optical tweezers to study cell wall mechanics is associated with transillumination technique of the trapped object position sensing, which prevents accurate mechanical testing in the proximity to the cell. In this work we use an optical tweezers in conjunction with a position-sensing system, which spectrally separates signals from the trapped fluorescent microsphere and imaging background. We have used this setup to study mechanics of the cell wall and PM separated from the underlying cytoskeleton on human embryonic kidney cells. We measured the force exerted by the cell on the trapped microsphere as a function of the cell wall displacement during the process of tether formation, and as a function of time during the process of tether growth and relaxation. Tethering force - cell wall displacement profiles have shown a behavior, implying that tether formation process starts with elastic deformation of the intact cell wall, followed by the plastic deformations and sliding of the PM over the underlying cytoskeleton, and ends with the local separation of a PM. Tethering force - cell wall displacement profiles have been used to estimate tether formation force, stiffness parameter of the cell wall and the works of tether formation, elastic and plastic deformations of the cell wall, related to the mechanical properties of a composite cell wall and cell wall - plasma membrane association strength. Temporal steady-state and relaxation tethering force profiles have been similar to the ones measured using transillumination position sensing, however average force values have been smaller in our case, due to the methodological differences. Our results demonstrate that measurements of cell wall and PM mechanical properties using optically-trapped fluorescent microspheres presents a versatile technology for studying of the cellular mechanics, especially effective in the proximity of the trapped microsphere to the cell.

Proceedings Article | 1 June 2004 Paper

Paper

Effect of salicylate on outer hair cell plasma membrane viscoelasticity: studies using optical tweezers

Sergey Ermilov, William Brownell, Bahman Anvari

Proceedings Volume 5331, (2004) https://doi.org/10.1117/12.529200

KEYWORDS: Phase modulation, Optical tweezers, Plasma, Cytoskeletons, Ferroelectric materials, Chlorine, Composites, Sodium, Kidney, Calibration

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Proceedings Article | 1 June 2004 Paper

Paper

Optical tweezers study of viscoelastic properties in the outer hair cell plasma membrane

David Murdock, Sergey Ermilov, Feng Qian, William Brownell, Bahman Anvari

Proceedings Volume 5331, (2004) https://doi.org/10.1117/12.529597

KEYWORDS: Plasma, Optical tweezers, Photodetectors, Data modeling, Neodymium, Phase modulation, Cytoskeletons, Mechanics, Calibration, Molecules

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Proceedings Article | 1 June 2004 Paper

Paper

Membrane tether formation from voltage-clamped outer hair cells using optical tweezers

Feng Qian, Sergey Ermilov, David Murdock, William Brownell, Bahman Anvari

Proceedings Volume 5331, (2004) https://doi.org/10.1117/12.528540

KEYWORDS: Optical tweezers, Plasma, Luminescence, Mirrors, Calibration, Electromechanical design, Photodetectors, Optical amplifiers, Objectives, Microscopes

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Proceedings Article | 12 September 2003 Paper

Paper

Studies of cochlear outer hair cell membrane mechanics using optical tweezers

David Murdock, Sergey Ermilov, William Brownell, Bahman Anvari

Proceedings Volume 4949, (2003) https://doi.org/10.1117/12.476469

KEYWORDS: Optical tweezers, Plasma, Photodetectors, Mechanics, Phase modulation, Cytoskeletons, Calibration, Kidney, Molecules, Microscopes

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Proceedings Article | 19 June 2003 Paper

Paper

Trapping-force calibration in biological applications of optical tweezers

Sergey Ermilov, Bahman Anvari

Proceedings Volume 4962, (2003) https://doi.org/10.1117/12.477849

KEYWORDS: Optical tweezers, Calibration, Ferroelectric materials, Fluid dynamics, Motion controllers, Photodetectors, Microscopes, Beam splitters, Detection theory, Optical calibration

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Proceedings Article | 13 June 2000 Paper

Paper

Light activation of the immune system part I: influence on G-class antibodies

Vladimir Zharov, Vladimir Gevondyan, Natalya Gevondyan, Sergey Ermilov, Alla Volynskaya

Proceedings Volume 3914, (2000) https://doi.org/10.1117/12.388075

KEYWORDS: Blood, Light emitting diodes, Optical filters, Proteins, Lamps, Patents, Absorption, Photodynamic therapy, Pathogens, Plasma

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Showing 5 of 67 publications

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