Mr. Tao Zhong Profile

Tao Zhong

at Fujian Normal Univ

SPIE Involvement:

Author

Publications (2)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 20 November 2024 Paper

Tumor-associated collagen signatures and their clinical stage correlations in breast cancer as revealed by multiphoton microscopy

Tao Zhong, Yihui Zheng, Zhijun Li, Xi Chen, Liqin Zheng, Jianhua Chen, Xiahui Han, Jianxin Chen

Proceedings Volume 13242, 132420G (2024) https://doi.org/10.1117/12.3041267

KEYWORDS: Collagen, Tumors, Breast cancer, Tissues, Second harmonic generation, Feature extraction, Multiphoton microscopy, Biological samples, Biological imaging

Read Abstract +

Breast cancer (BC) is a significant health concern for women, with its classification into multiple stages contingent upon the dimensions of the tumor, the extent of lymph node involvement, and the presence of distant metastasis. Despite the application of uniform treatment protocols to cases of similar staging, the outcomes are subject to variability due to the inherent heterogeneity of the disease, highlighting an urgent need for further investigation. The tumor microenvironment (TME) plays a pivotal role in tumor progression and metastasis, with collagen fibers emerging as a critical component of the TME that is implicated in these processes. However, the precise interplay between collagen fibers and tumor staging remains to be elucidated. Advancements in multiphoton microscopy (MPM), which capitalizes on nonlinear optical phenomena, have yielded impressive imaging capabilities, facilitating the real-time visualization of tumor histology and the quantification of metabolic activity within tumors. Recent studies have underscored the intricate relationship between collagen fibers and the dynamics of tumor evolution.

In this study, we utilized multiphoton microscopy to image three distinct tumor-associated collagen signatures (TACS) at the invasive front of the tumor. We then used MATLAB to extract the corresponding collagen morphological features and analyzed their correlation with clinical staging. Our results revealed significant changes in the morphological features of collagen fibers in TACS across different stages of BC at the tumor invasion front. Notably, the proportionate area and number of collagen fibers were found to be inversely correlated with the clinical staging risk group of the disease. Our findings offer new perspectives for the clinical staging of BC, providing valuable insights that may enhance the predictive accuracy of disease progression and prognostic outcomes.

Proceedings Article | 27 November 2023 Paper

Monitoring changes of collagen fibers surrounding breast ductal carcinoma in situ using multiphoton microscopy

Xi Chen, Zhijun Li, Zheng Zhang, Xiong Zhang, Tao Zhong, Shuoyu Xu, Biying Yu, Jianxin Chen, Deyong Kang, Liqin Zheng

Proceedings Volume 12770, 127700W (2023) https://doi.org/10.1117/12.2687473

KEYWORDS: Collagen, Second harmonic generation, Breast, Tumors, Breast cancer, Multiphoton microscopy, Biological imaging

Read Abstract +

Ductal carcinoma in situ (DCIS) accounts for approximately 20% of all breast cancer. DCIS is a form of breast cancer that is restricted to the ducts and has not invaded surrounding breast tissue or spread to lymph nodes or other parts of the body. The grades of DCIS are classified as low, intermediate, and high, based on cytonuclear features, and high-grade DCIS has a higher risk of progressing into invasive ductal carcinoma (IDC). The collagen fibers are an important component of the tumor microenvironment (TME) in DCIS and play an important role in tumor formation and progression. Multiphoton microscopy (MPM) based on second harmonic generation (SHG) and two-photon excitation fluorescence (TPEF) can monitor the morphological changes of collagen fibers around DCIS. SHG is currently considered the gold standard for visualizing collagen fibers and has been widely employed in various cancer-related studies of collagen fibers. Our investigation employed MPM imaging of breast tissue to observe the differences in collagen fibers within three distinct grades of DCIS. Through image processing, we were able to quantify various attributes of collagen fibers enveloping DCIS lesions of varying grades. The study found that collagen fibers surrounding low-grade DCIS were denser and exhibited more sinuous shapes, whereas collagen fibers around intermediate and high-grade DCIS lesions were less dense and exhibited a more organized arrangement. The study suggests that MPM imaging is a powerful tool for investigating the microenvironment of DCIS and may provide valuable information for predicting disease progression and prognosis.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years