The structural characteristics of bile duct tissue can indirectly reflect the physiological function of the bile duct, so understanding the pathological process of bile duct tissue can improve the prognosis of intrahepatic cholangiocarcinoma. However, the existing technology cannot accurately and quickly determine the stage of the bile duct tissue lesion, which will have a certain impact on the treatment. In this study, normal bile ducts, inflammatory bile ducts, and intrahepatic cholangiocarcinoma were distinguished using label-free multiphoton microscopy (MPM). The experimental results show that high-resolution images can clearly distinguish normal bile duct, inflammatory bile duct and intrahepatic cholangiocarcinoma through cell morphology and tissue structure. Therefore, MPM can be used as an effective optical tool for the diagnosis of intrahepatic cholangiocarcinoma in unstained histological sections. It is expected in the future that MPM can play a greater role in the clinic.
The mortality rate of gastric cancer ranks second in the world. The prognosis of early gastric cancer is good, while the prognosis of advanced gastric cancer is poor. Therefore, early diagnosis of gastric cancer is the key to determining the prognosis of patients. Traditional pathological analysis takes a long time, and the results may have some subjectivity and randomness. Therefore, we propose a method for distinguishing normal and early gastric cancer sites by multiphoton microscopy combined with acridine orange rapid staining. The experimental results confirmed that multiphoton microscopy combined with acridine orange staining can effectively describe the normal microstructure of the human gastric mucosa,and these results laid the experimental foundation for the establishment of clinical diagnostic criteria for gastric cancer.
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