Fluorescence-enhanced europium complexes for the assessment of renal function

Lori K. Chinen; Karen P. Galen; K. T. Kuan; Mary E. Dyszlewski; Hiroaki Ozaki; Hiroaki Sawai; Raghootama S. Pandurangi; Frederick G. Jacobs; Richard B. Dorshow; Raghavan Rajagopalan

doi:10.1117/12.763665

22 February 2008 Fluorescence-enhanced europium complexes for the assessment of renal function

Lori K. Chinen, Karen P. Galen, K. T. Kuan, Mary E. Dyszlewski, Hiroaki Ozaki, Hiroaki Sawai, Raghootama S. Pandurangi, Frederick G. Jacobs, Richard B. Dorshow, Raghavan Rajagopalan

Author Affiliations +

Proceedings Volume 6867, Molecular Probes for Biomedical Applications II; 68670B (2008) https://doi.org/10.1117/12.763665
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Real-time, non-invasive assessment of glomerular filtration rate (GFR) is essential not only for monitoring critically ill patients at the bedside, but also for staging and monitoring patients with chronic kidney disease. In our pursuit to develop exogenous luminescent probes for dynamic optical monitoring of GFR, we have prepared and evaluated Eu³⁺ complexes of several diethylenetriamine pentaacetate (DTPA)-monoamide ligands bearing molecular "antennae" to enhance metal fluorescence via the intramolecular ligand-metal fluorescence resonance energy transfer (FRET) process. The results show that Eu-DTPA-monoamide complex 13a, which contains a quinoxanlinyl antenna, exhibits large (c.a. 2700-fold) Eu³⁺ fluorescence enhancement over Eu-DTPA (4c). Indeed, complex 13a exhibits the highest fluorescent enhancement observed thus far in the DTPA-type metal complexes. The renal clearance profile of the corresponding radioactive ¹¹¹In complex 13c is similar to that of ¹¹¹In-DTPA, albeit 13c clears slower than ¹¹¹In-DTPA. The biodistribution data indicates that 13c, and, by inference, 13a clear via a complex mechanism that includes glomerular filtration.

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Lori K. Chinen, Karen P. Galen, K. T. Kuan, Mary E. Dyszlewski, Hiroaki Ozaki, Hiroaki Sawai, Raghootama S. Pandurangi, Frederick G. Jacobs, Richard B. Dorshow, and Raghavan Rajagopalan "Fluorescence-enhanced europium complexes for the assessment of renal function", Proc. SPIE 6867, Molecular Probes for Biomedical Applications II, 68670B (22 February 2008); https://doi.org/10.1117/12.763665

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